National Institute of Public Health - NIH, Poland.
Brief Funct Genomics. 2018 Nov 26;17(6):471-476. doi: 10.1093/bfgp/elx033.
Modern diagnostics is in general based on molecular biology methods. Nowadays sequencing-based methods, especially whole genome sequencing, are becoming increasingly important. Implementation of such methods into routine diagnostic of highly dangerous pathogens, like Bacillus anthracis, Francisella tularensis, Yersinia pestis, Ebola virus, MERS, Lassa virus etc. would be very helpful. The best diagnostic strategy would be the metagenomic sequencing directly from the clinical sample. Implementation of majority of currently available WGS platforms inside the BSL-3 or 4 laboratory is impractical because of the size of the equipment and time consuming wet lab part (e.g. library preparation). Nowadays there is a possibility to implement pocket size MinION - real time whole genome sequencer into BSL-3 and 4 laboratory for rapid and precise diagnostic purposes.
现代诊断学通常基于分子生物学方法。如今，基于测序的方法，特别是全基因组测序，变得越来越重要。将这些方法应用于炭疽杆菌、土拉弗朗西斯菌、鼠疫耶尔森菌、埃博拉病毒、MERS、拉萨病毒等高危险病原体的常规诊断中非常有帮助。最好的诊断策略是直接从临床样本中进行宏基因组测序。由于设备的体积和耗时的湿实验室部分（例如文库制备），将大多数现有的 WGS 平台在 BSL-3 或 4 实验室中实施是不切实际的。如今，有可能将口袋大小的 MinION——实时全基因组测序仪引入 BSL-3 和 4 实验室，用于快速准确的诊断目的。
