Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Japan.
Faculty of Medicine, Sam Ratulangi University, Manado, Indonesia.
Adv Exp Med Biol. 2019;1129:143-150. doi: 10.1007/978-981-13-6037-4_10.
DNA sequencing has reached an unprecedented level with the advent of Oxford Nanopore Technologies' MinION. The low equipment investment, ease of library preparation, small size, and powered only by a laptop computer enable the portability for on-site sequencing. MinION has had its role in clinical, biosecurity, and environmental fields. Here, we describe the many facets of on-site sequencing with MinION. First, we will present some field works using MinION. We will discuss the requirements for targeted or whole genome sequencing and the challenges faced by each technique. We will also elaborate the bioinformatics procedures available for data analysis in the field. MinION has greatly changed the way we do sequencing by bringing the sequencer closer to the biodiversity. Although numerous limitations exist for MinION to be truly portable, improvements of procedures and equipment will enhance MinION's role in the field.
DNA 测序随着牛津纳米孔技术的 MinION 的出现达到了前所未有的水平。低设备投资、文库制备简便、体积小，仅由笔记本电脑供电，实现了现场测序的便携性。MinION 在临床、生物安全和环境领域都有其作用。在这里，我们描述了使用 MinION 进行现场测序的多个方面。首先，我们将介绍一些使用 MinION 的现场工作。我们将讨论靶向或全基因组测序的要求以及每种技术所面临的挑战。我们还将详细介绍现场数据分析可用的生物信息学程序。MinION 通过将测序仪更接近生物多样性，极大地改变了我们的测序方式。尽管 MinION 要真正实现便携性还存在许多限制，但程序和设备的改进将增强 MinION 在该领域的作用。
