Allergy Unit, Instituto de Investigación Biomédica de Málaga (IBIMA)-Regional University Hospital of Málaga, Malaga, Spain.
Research Laboratory-Allergy Unit, IBIMA-Regional University Hospital of Málaga, Málaga, Spain.
Int Forum Allergy Rhinol. 2018 Jan;8(1):15-19. doi: 10.1002/alr.22039. Epub 2017 Nov 14.
The measurement of nasal specific IgE (NsIgE) in local allergic rhinitis (LAR) patients is challenging and shows variability. The objective of this work was to evaluate a minimally-invasive method of direct detection of NsIgE in patients with LAR to Dermatophagoides pteronyssinus (DP) using an automated immunoassay.
Fifty patients participated (LAR, n = 14; allergic rhinitis (AR), n = 20; healthy controls [HC], n = 16). Detection of NsIgE was performed by direct application of the solid phase of a commercial DP ImmunoCAP® test 24 hours after DP nasal provocation.
There was no difference in the median volume of secretion absorbed by the solid phase of the ImmunoCAP test in the 3 studied groups (p = 0.17). According to receiver operating characteristic (ROC) curve analysis, NsIgE ≥0.1450 was the optimal cutoff point, obtaining in LAR patients 42.86% sensitivity with the highest specificity (100%), and 75% sensitivity and 100% specificity for AR.
This study demonstrates the detection of NsIgE to DP in LAR by using a simple, commercial device with high specificity.
局部过敏性鼻炎 (LAR) 患者的鼻特异性 IgE（NsIgE）测量具有挑战性且存在变异性。本研究的目的是评估一种使用自动化免疫测定法直接检测对屋尘螨 (DP) 呈阳性的 LAR 患者 NsIgE 的微创方法。
共有 50 名患者参与（LAR 组，n=14；过敏性鼻炎组，n=20；健康对照组，n=16）。DP 鼻腔激发后 24 小时，直接应用商业 DP ImmunoCAP® 试验的固相检测 NsIgE。
在 3 个研究组中，固相吸收的分泌量中位数没有差异（p=0.17）。根据受试者工作特征（ROC）曲线分析，NsIgE≥0.1450 是最佳截断点，LAR 患者的灵敏度为 42.86%，特异性最高（100%），AR 的灵敏度为 75%，特异性为 100%。
本研究证明了使用简单的商业设备可检测 LAR 患者的 DP 特异性 IgE，特异性高。
