直接经鼻腔应用 ImmunoCAP ®固相可增加局部变应性鼻炎患者鼻内特异性免疫球蛋白 E 的检测。
Direct intranasal application of the solid phase of ImmunoCAP® increases nasal specific immunoglobulin E detection in local allergic rhinitis patients.
机构信息
Allergy Unit, Instituto de Investigación Biomédica de Málaga (IBIMA)-Regional University Hospital of Málaga, Malaga, Spain.
Research Laboratory-Allergy Unit, IBIMA-Regional University Hospital of Málaga, Málaga, Spain.
出版信息
Int Forum Allergy Rhinol. 2018 Jan;8(1):15-19. doi: 10.1002/alr.22039. Epub 2017 Nov 14.
BACKGROUND
The measurement of nasal specific IgE (NsIgE) in local allergic rhinitis (LAR) patients is challenging and shows variability. The objective of this work was to evaluate a minimally-invasive method of direct detection of NsIgE in patients with LAR to Dermatophagoides pteronyssinus (DP) using an automated immunoassay.
METHODS
Fifty patients participated (LAR, n = 14; allergic rhinitis (AR), n = 20; healthy controls [HC], n = 16). Detection of NsIgE was performed by direct application of the solid phase of a commercial DP ImmunoCAP® test 24 hours after DP nasal provocation.
RESULTS
There was no difference in the median volume of secretion absorbed by the solid phase of the ImmunoCAP test in the 3 studied groups (p = 0.17). According to receiver operating characteristic (ROC) curve analysis, NsIgE ≥0.1450 was the optimal cutoff point, obtaining in LAR patients 42.86% sensitivity with the highest specificity (100%), and 75% sensitivity and 100% specificity for AR.
CONCLUSION
This study demonstrates the detection of NsIgE to DP in LAR by using a simple, commercial device with high specificity.
背景
局部过敏性鼻炎 (LAR) 患者的鼻特异性 IgE（NsIgE）测量具有挑战性且存在变异性。本研究的目的是评估一种使用自动化免疫测定法直接检测对屋尘螨 (DP) 呈阳性的 LAR 患者 NsIgE 的微创方法。
方法
共有 50 名患者参与（LAR 组，n=14；过敏性鼻炎组，n=20；健康对照组，n=16）。DP 鼻腔激发后 24 小时，直接应用商业 DP ImmunoCAP® 试验的固相检测 NsIgE。
结果
在 3 个研究组中，固相吸收的分泌量中位数没有差异（p=0.17）。根据受试者工作特征（ROC）曲线分析，NsIgE≥0.1450 是最佳截断点，LAR 患者的灵敏度为 42.86%，特异性最高（100%），AR 的灵敏度为 75%，特异性为 100%。
结论
本研究证明了使用简单的商业设备可检测 LAR 患者的 DP 特异性 IgE，特异性高。
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