Professor Massoumi Laboratory, Department of Chemistry, College of Sciences, Shiraz University, Shiraz 71454, Iran.
Professor Massoumi Laboratory, Department of Chemistry, College of Sciences, Shiraz University, Shiraz 71454, Iran.
Talanta. 2018 Feb 1;178:870-878. doi: 10.1016/j.talanta.2017.10.005. Epub 2017 Oct 18.
A new, fast and inexpensive colorimetric sensor was developed for chiral recognition of tryptophan enantiomers using chitosan-capped silver nanoparticles. The function of the sensor was based on scanometry and spectrophotometry of the colored product of a reaction solution containing a mixture of chitosan-capped silver nanoparticles, phosphate buffer and tryptophan enantiomers. The image of the colored solution was taken using the scanometer and the corresponding color values were obtained using Photoshop software which subsequently were used for optimization of the experimental parameters as the analytical signal. Two types of color values system were investigated: RGB (red, green and blue values) and CMYK (cyan, magenta, yellow and black values). The color values indicated that L-tryptophan had better interaction than D-tryptophan with chitosan-capped silver nanoparticles. A linear relationship between the analytical signal and the concentration of L-tryptophan was obtained in the concentration range of 1.3 × 10-4.6 × 10molL. Detection limits, were obtained to be 2.1 × 10, 2.4 × 10 and 3.8 × 10molL for L-tryptophan based on R (red), G (green) and B (blue) values, respectively.
一种新的、快速且廉价的比色传感器，使用壳聚糖包覆的银纳米粒子对手性识别色氨酸对映体进行了开发。该传感器的功能基于扫描计和反应溶液的分光光度法，反应溶液包含壳聚糖包覆的银纳米粒子、磷酸盐缓冲液和色氨酸对映体的混合物。使用扫描计拍摄有色溶液的图像，并使用 Photoshop 软件获得相应的颜色值，随后将其用于优化实验参数作为分析信号。研究了两种类型的颜色值系统：RGB（红色、绿色和蓝色值）和 CMYK（青色、品红色、黄色和黑色值）。颜色值表明，L-色氨酸与壳聚糖包覆的银纳米粒子的相互作用优于 D-色氨酸。在 1.3×10-4.6×10molL 的浓度范围内，获得了分析信号与 L-色氨酸浓度之间的线性关系。基于 R（红色）、G（绿色）和 B（蓝色）值，分别获得了 L-色氨酸的检测限为 2.1×10、2.4×10 和 3.8×10molL。
