University of Sao Paulo, Ribeirao Preto, Brasil.
Physiol Res. 2018 Mar 16;67(1):107-115. doi: 10.33549/physiolres.933614. Epub 2017 Nov 10.
The main aim of the present investigation was to verify the effects of three overtraining (OT) protocols performed in downhill (OTR/down), uphill (OTR/up) and without inclination (OTR) on the protein levels of Akt (Ser473), AMPKalpha (Thr172), PGC-1alpha, plasma membrane GLUT-1 and GLUT-4 as well as on the glycogen contents in mice gastrocnemius. A trained (TR) protocol was used as positive control. Rodents were divided into naive (N, sedentary mice), control (CT, sedentary mice submitted to the performance evaluations), TR, OTR/down, OTR/up and OTR groups. At the end of the experimental protocols, gastrocnemius samples were removed and used for immunoblotting analysis as well as for glycogen measurements. There was no significant difference between the experimental groups for the protein levels of pAkt (Ser473), pAMPKalpha (Thr172), PGC-1alpha, plasma membrane GLUT-1 and GLUT-4. However, the OTR/up protocol exhibited higher contents of glycogen compared to the CT and TR groups. In summary, the OTR/up group increased the gastrocnemius glycogen content without significant changes of pAkt (Ser473), pAMPKalpha (Thr172), PGC-1alpha, plasma membrane GLUT-1 and GLUT-4.
本研究的主要目的是验证三种下坡（OTR/down）、上坡（OTR/up）和无倾斜（OTR）过度训练方案对 Akt（Ser473）、AMPKalpha（Thr172）、PGC-1alpha、质膜 GLUT-1 和 GLUT-4 的蛋白水平以及小鼠比目鱼肌糖原含量的影响。采用训练（TR）方案作为阳性对照。将啮齿动物分为未训练（N，久坐不动的小鼠）、对照组（CT，接受性能评估的久坐不动的小鼠）、TR、OTR/down、OTR/up 和 OTR 组。在实验方案结束时，取出比目鱼肌样本，用于免疫印迹分析和糖原测量。pAkt（Ser473）、pAMPKalpha（Thr172）、PGC-1alpha、质膜 GLUT-1 和 GLUT-4 的蛋白水平在实验组之间无显著差异。然而，与 CT 和 TR 组相比，OTR/up 方案的糖原含量更高。总之，OTR/up 组增加了比目鱼肌糖原含量，而 pAkt（Ser473）、pAMPKalpha（Thr172）、PGC-1alpha、质膜 GLUT-1 和 GLUT-4 没有明显变化。
