Department of Gastrointestinal Surgery, Affiliated Hospital of Jining Medical UniversityJiningP.R. China.
Oncol Res. 2018 Apr 10;26(3):473-481. doi: 10.3727/096504017X15105708598531. Epub 2017 Nov 14.
The long noncoding RNA HOX transcript antisense RNA (HOTAIR) has been found to be overexpressed in many human malignancies and involved in tumor progression and metastasis. Although the downstream target through which HOTAIR modulates tumor metastasis is not well known, evidence suggests that microRNA-197 (miR-197) might be involved in this event. In the present study, the significance of HOTAIR and miR-197 in the progression of colorectal cancer was detected in vitro and in vivo. We found that HOTAIR expression was significantly increased in colorectal cancer cells and tissues. In contrast, the expression of miR-197 was obviously decreased. We further demonstrated that HOTAIR knockdown promoted apoptosis and inhibited cell proliferation, migration, and invasion in vitro and in vivo. Moreover, HOTAIR modulated the progression of colorectal cancer by competitively binding miR-197. Taken together, our study has identified a novel pathway through which HOTAIR exerts its oncogenic role and provided a molecular basis for potential applications of HOTAIR in the prognosis and treatment of colorectal cancer.
长链非编码 RNA HOX 转录反义 RNA（HOTAIR）已被发现过度表达于许多人类恶性肿瘤中，并参与肿瘤的进展和转移。尽管 HOTAIR 调节肿瘤转移的下游靶标尚不清楚，但有证据表明 microRNA-197（miR-197）可能参与了这一事件。在本研究中，我们在体外和体内检测了 HOTAIR 和 miR-197 在结直肠癌进展中的意义。我们发现 HOTAIR 在结直肠癌细胞和组织中的表达明显增加，而 miR-197 的表达明显降低。我们进一步证明，HOTAIR 敲低可促进细胞凋亡，并抑制细胞增殖、迁移和侵袭，无论是在体外还是体内。此外，HOTAIR 通过竞争性结合 miR-197 来调节结直肠癌的进展。综上所述，我们的研究确定了 HOTAIR 发挥致癌作用的新途径，并为 HOTAIR 在结直肠癌的预后和治疗中的潜在应用提供了分子基础。
