Département de biochimie, de microbiologie et de bio-informatique, Université Laval, Québec, Québec, G1V 0A6, Canada; Centre d'études nordiques (CEN), Université Laval, Québec, Québec, G1V 0A6, Canada; Institut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Québec, Québec, G1V 0A6, Canada; Takuvik, Unité Mixte Interntionale (UMI 3376) Université Laval (Canada) & Centre National de la Recherche Scientifique (France), Québec QC GIV 0A6, Canada.
Virus Res. 2018 Jan 15;244:84-89. doi: 10.1016/j.virusres.2017.11.008. Epub 2017 Nov 11.
RNA viruses that infect microbes are now recognized as an active, persistent and important component of the aquatic microbial community. While some information about the diversity and dynamics of the RNA virioplankton has been derived from culture-based and single gene approaches, research based on viromic and metatransciptomic methods has generated unprecedented insight into this relatively understudied class of microbes. Here, the relevant literature is summarized and discussed, including viromic studies of extracellular aquatic RNA viral assemblages, and transcriptomic studies of active and associated RNA viruses from aquatic environments followed by commentary on the present challenges and future directions of this field of research.
感染微生物的 RNA 病毒现在被认为是水生微生物群落中活跃、持久和重要的组成部分。虽然通过基于培养和单基因的方法已经获得了一些有关 RNA 病毒浮游生物多样性和动态的信息，但基于病毒组学和宏转录组学方法的研究为这一相对研究较少的微生物类群提供了前所未有的深入了解。本文对相关文献进行了总结和讨论，包括对水生环境中细胞外 RNA 病毒组合的病毒组学研究，以及对水生环境中活跃和相关 RNA 病毒的转录组学研究，并对该研究领域当前的挑战和未来方向进行了评论。
