Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, Taipei, Taiwan.
Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan.
Sci Rep. 2017 Nov 14;7(1):15524. doi: 10.1038/s41598-017-15916-2.
The purpose of this study was to investigate the neuroprotective effects of low-intensity pulsed ultrasound (LIPUS) on behavioral and histological outcomes in a mouse model of traumatic brain injury (TBI). Mice subjected to controlled cortical impact injury were treated with LIPUS in the injured region daily for a period of 4 weeks. The effects of LIPUS on edema were observed by MR imaging in the mouse brain at 1 and 4 days following TBI. Brain water content, blood-brain barrier permeability, histology analysis, and behavioral studies were performed to assess the effects of LIPUS. Two-way analysis of variance and Student t test were used for statistical analyses, with a significant level of 0.05. Treatment with LIPUS significantly attenuated brain edema, blood-brain barrier permeability, and neuronal degeneration beginning at day 1. Compared with the TBI group, LIPUS also significantly improved functional recovery and reduced contusion volumes up to post-injury day 28. Post-injury LIPUS treatment reduced brain edema and improved behavioral and histological outcomes following TBI. The neuroprotective effects of LIPUS may be a promising new technique for treating TBI.
本研究旨在探讨低强度脉冲超声（LIPUS）对创伤性脑损伤（TBI）小鼠模型行为和组织学结果的神经保护作用。对接受皮质撞击伤的小鼠在损伤区域进行每日 LIPUS 治疗，持续 4 周。在 TBI 后 1 天和 4 天，通过磁共振成像观察 LIPUS 对脑水肿的影响。通过脑水含量、血脑屏障通透性、组织学分析和行为研究来评估 LIPUS 的作用。采用方差分析和 t 检验进行统计学分析，显著性水平为 0.05。LIPUS 治疗从第 1 天开始显著减轻脑水肿、血脑屏障通透性和神经元变性。与 TBI 组相比，LIPUS 还显著改善了功能恢复，并在损伤后 28 天减少了挫伤体积。损伤后 LIPUS 治疗可减轻脑水肿，并改善 TBI 后的行为和组织学结果。LIPUS 的神经保护作用可能是治疗 TBI 的一种有前途的新技术。
