Department of Magnetic Resonance Imaging, Qinhuangdao Municipal No. 1 Hospital, Qinhuangdao, China.
Department of Radiology, Peking University Third Hospital, Beijing, China.
Aging (Albany NY). 2024 Aug 12;16(15):11744-11754. doi: 10.18632/aging.206060.
To evaluate the protective effect of LIPUS at the early stage of brain trauma in rats, 45 rats were randomly divided into 3 groups: sham ( = 15), TBI ( = 15) and LIPUS treatment groups ( = 15). Ipsilateral and contralateral cortical and thalamic parameters obtained by diffusion tensor imaging (DTI) and fast low-angle shot magnetic resonance imaging (FLASH-MRI) were measured at different times after trauma. For fractional anisotropy (FA) and T2 values, two-way repeated measures ANOVA with Tukey's post hoc was used for intergroup comparisons. With observation time prolonged, the FA values of the ipsilateral cortex in the TBI group gradually increased and were significantly higher than those in the LIPUS treatment group on Day 7 (adjusted = 0.0067). FA values in the contralateral cortex decreased at this time and were significantly lower than those in the LIPUS treatment group (adjusted = 0.0192). Meanwhile, compared with LIPUS group, FA values were significantly higher in the injured thalamus (adjusted = 0.0025). Combined with correlation analysis, FA values were positively correlated with neuronal damage ( = 0.0148, r = 0.895). At 7 days after trauma, T2 values in the ipsilateral cortex of the TBI group were significantly lower. After analysis of ferritin content and correlation, we found that T2 values were negatively correlated with ferritin ( = 0.0259, r = -0.849). By measuring post-traumatic changes in FA and T2 values, it is possible to demonstrate a neuronal protective effect of LIPUS in the early phase of TBI rats and promote brain rehabilitation.
为了评估低强度脉冲超声（LIPUS）在大鼠脑创伤早期的保护作用，将 45 只大鼠随机分为 3 组：假手术组（n=15）、创伤组（n=15）和 LIPUS 治疗组（n=15）。在创伤后不同时间点，通过弥散张量成像（DTI）和快速小角度激发磁共振成像（FLASH-MRI）测量对侧和同侧皮质及丘脑参数。对于各向异性分数（FA）和 T2 值，采用双因素重复测量方差分析和 Tukey 事后检验进行组间比较。随着观察时间的延长，创伤组大鼠对侧皮质 FA 值逐渐升高，在第 7 天明显高于 LIPUS 治疗组（校正后 P=0.0067）。此时对侧皮质 FA 值降低，明显低于 LIPUS 治疗组（校正后 P=0.0192）。同时，与 LIPUS 组相比，损伤丘脑的 FA 值明显升高（校正后 P=0.0025）。结合相关性分析，FA 值与神经元损伤呈正相关（r=0.895，P=0.0148）。在创伤后 7 天，创伤组大鼠对侧皮质 T2 值明显降低。通过分析铁蛋白含量和相关性，我们发现 T2 值与铁蛋白呈负相关（r=-0.849，P=0.0259）。通过测量创伤后 FA 和 T2 值的变化，可以证明 LIPUS 在大鼠脑创伤早期具有神经元保护作用，并促进脑康复。
