慢性阻塞性肺疾病（COPD）患者外周血单核细胞（PBMC）中SIRT1和FOXO1信使核糖核酸（mRNA）的表达与身体活动相关。

SIRT1 and FOXO1 mRNA expression in PBMC correlates to physical activity in COPD patients.

作者信息

Taka Chihiro, Hayashi Ryuji, Shimokawa Kazuki, Tokui Kotaro, Okazawa Seisuke, Kambara Kenta, Inomata Minehiko, Yamada Toru, Matsui Shoko, Tobe Kazuyuki

机构信息

First Department of Internal Medicine, Faculty of Medicine, University of Toyama, Sugitani, Toyama, Toyama, Japan.

出版信息

Int J Chron Obstruct Pulmon Dis. 2017 Nov 3;12:3237-3244. doi: 10.2147/COPD.S144969. eCollection 2017.

BACKGROUND

Physical activity (PA) is considered as one of the most important prognostic predictors in chronic obstructive pulmonary disease (COPD) patients. Longevity gene, , is reported to be involved in the pathogenesis of COPD by regulating the signaling pathways of oxidative stress, inflammation, and aging. We hypothesize that SIRT1 and related genes are also associated with the benefits of PA in COPD patients.

METHODS

Eighteen COPD outpatients were enrolled in this study, and their PA level was assessed with an accelerometer. We assessed the and related genes mRNA expression levels in the peripheral blood mononuclear cells (PBMCs) of the subjects. We carried out respiratory function testing, blood gas analysis, the 6-minute walk test, and measurement of the cross-sectional area of the erector spinae muscles (ESMCSA) by chest computed tomography. We analyzed the association of PA with the results of each of the examinations.

RESULTS

The mean age was 72±9 years, and the mean forced expiratory volume in 1 second was 1.4±0.56 L (52%±19% predicted). Our findings revealed a correlation between the daily PA and ESMCSA. The and mRNA expression levels in PBMCs were positively correlated with moderate-PA time (=0.60, =0.008 for and =0.59, =0.01 for ).

背景

体力活动（PA）被认为是慢性阻塞性肺疾病（COPD）患者最重要的预后预测指标之一。据报道，长寿基因SIRT1通过调节氧化应激、炎症和衰老的信号通路参与COPD的发病机制。我们假设SIRT1及相关基因也与PA对COPD患者的益处相关。

方法

本研究纳入了18名COPD门诊患者，并用加速度计评估他们的PA水平。我们评估了受试者外周血单个核细胞（PBMC）中SIRT1及相关基因的mRNA表达水平。我们进行了呼吸功能测试、血气分析、6分钟步行试验，并通过胸部计算机断层扫描测量竖脊肌横截面积（ESMCSA）。我们分析了PA与各项检查结果之间的关联。

结果

平均年龄为72±9岁，平均第1秒用力呼气量为1.4±0.56L（占预计值的52%±19%）。我们的研究结果显示每日PA与ESMCSA之间存在相关性。PBMC中SIRT1和PGC-1αmRNA表达水平与中度PA时间呈正相关（SIRT1：r = 0.60，P = 0.008；PGC-1α：r = 0.59，P = 0.01）。