Department of Emergency Medicine, General Hospital of Chinese People's Armed Police Forces, Beijing 100039, China.
State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China.
Future Microbiol. 2017 Dec;12:1511-1522. doi: 10.2217/fmb-2017-0072. Epub 2017 Nov 15.
This study dealt with genomic characterization of type 1 IncC resistance plasmids, capable of spreading across taxonomic borders, from China.
MATERIALS & METHODS: p112298-tetA was sequenced and compared with type 1 IncC reference plasmid pR148 and two available sequenced type 1 IncC plasmids pHS36-NDM and pVAS3-1 from China.
These plasmids contained one or more exogenous resistance islands, which included the ARI-A islands, the ARI-B islands, the ISEcp1-bla units and the bla region and were inserted at various sites in the IncC backbone and thus represented three distinct lineages.
Complex rearrangement and homologous recombination events have occurred during evolution of p112298-tetA, making it significantly differ modularly from the other three plasmids with respect to both plasmid backbone and exogenous resistance regions.
本研究涉及能够跨越分类边界传播的 1 型 IncC 耐药质粒的基因组特征，这些质粒来自中国。
对 p112298-tetA 进行测序，并与 1 型 IncC 参考质粒 pR148 以及来自中国的两个已测序的 1 型 IncC 质粒 pHS36-NDM 和 pVAS3-1 进行比较。
这些质粒包含一个或多个外源性耐药岛，包括 ARI-A 岛、ARI-B 岛、ISEcp1-bla 单元和 bla 区，并插入 IncC 骨架的不同部位，因此代表了三个不同的谱系。
p112298-tetA 在进化过程中发生了复杂的重排和同源重组事件，使其在质粒骨架和外源性耐药区方面与其他三个质粒在模块上有显著差异。
