Ouyang Jing, Sun Fengjun, Zhou Dongsheng, Feng Jiao, Zhan Zhe, Xiong Zhijian, Yang Bo, Liu Zhirui, Li Tingbo, Tong Yigang, Xia Peiyuan
Department of Pharmacy, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China,
Department of Pharmacy, Chongqing Public Health Medical Center, Chongqing 400038, China.
Infect Drug Resist. 2018 Sep 12;11:1447-1460. doi: 10.2147/IDR.S165818. eCollection 2018.
Plasmid-mediated multi-drug resistance (MDR) has been widely found in . P10159 was isolated from a human case of postoperative urinary tract infection in a Chinese teaching hospital.
The complete nucleotide sequences of five resistance plasmids pP10159-1, pP10159-2, pP10159-3, pP10159-4 and pP10159-5 from P10159 were determined through high-throughput genome sequencing, and then compared with related plasmids sequences. Plasmid transfer, CarbaNP test of carbapenemase activity, and bacterial antimicrobial susceptibility test were performed to characterize resistance phenotypes mediated by these plasmids.
pP10159-1 carrying and pP10159-2 harboring plus were almost identical to IncX3 plasmid pNDM-HN380 and IncN1 plasmid pP378-IMP, respectively. The -carrying plasmids pP10159-3, pHS062105-3 and pECN49-KPC were highly similar to each other, and constituted a novel group of plasmids belonging to an unknown incomparability group. The MDR plasmids pP10159-4 and pP10159-5 had the backbones highly similar to IncHI4 plasmid pNDM-CIT and type 2 IncC plasmid pR55, respectively, but their accessory resistance regions differed from pNDM-CIT and pR55, respectively. The five plasmids from the P10159 isolate contained a total of 24 different genes or gene loci, which contributed to resistance to 13 distinct antibiotic molecules or toxic compounds.
This is the first report of co-occurrence of five different resistance plasmids, with determination of their complete sequences. Data presented here provide a deeper insight into co-selection and maintenance of multiple plasmids and an extremely large number of resistance genes in a single bacterial isolate.
质粒介导的多重耐药性（MDR）已在……中广泛发现。P10159是从中国一家教学医院的一名术后尿路感染患者中分离得到的。
通过高通量基因组测序确定了来自P10159的5个耐药质粒pP10159 - 1、pP10159 - 2、pP10159 - 3、pP10159 - 4和pP10159 - 5的完整核苷酸序列，然后与相关质粒序列进行比较。进行了质粒转移、碳青霉烯酶活性的CarbaNP试验以及细菌药敏试验，以表征这些质粒介导的耐药表型。
携带……的pP10159 - 1和携带……加……的pP10159 - 2分别与IncX3质粒pNDM - HN380和IncN1质粒pP378 - IMP几乎相同。携带……的质粒pP10159 - 3、pHS062105 - 3和pECN49 - KPC彼此高度相似，并构成了一组属于未知不相容群的新型质粒。多重耐药质粒pP10159 - 4和pP10159 - 5的骨架分别与IncHI4质粒pNDM - CIT和2型IncC质粒pR55高度相似，但它们的辅助耐药区域分别与pNDM - CIT和pR55不同。来自P10159分离株的这5个质粒总共包含24个不同的基因或基因位点，这些基因或基因位点导致对13种不同的抗生素分子或有毒化合物产生耐药性。
这是首次报道同时出现5种不同的耐药质粒并测定其完整序列。此处呈现的数据为深入了解单个细菌分离株中多种质粒和大量耐药基因的共同选择及维持提供了见解。
