Lei Chang-Wei, Kong Ling-Han, Ma Su-Zhen, Liu Bi-Hui, Chen Yan-Peng, Zhang An-Yun, Wang Hong-Ning
College of Life science, Sichuan University, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, 985 Project Science Innovative Platform for Resource and Environment Protection of Southwestern China, Chengdu, People's Republic of China.
College of Life science, Sichuan University, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, 985 Project Science Innovative Platform for Resource and Environment Protection of Southwestern China, Chengdu, People's Republic of China.
Plasmid. 2017 Sep;93:1-5. doi: 10.1016/j.plasmid.2017.07.002. Epub 2017 Jul 27.
IncC plasmids are of great concern as vehicles of broad-spectrum cephalosporins and carbapenems resistance genes bla and bla. The aim of this study was to sequence and characterize a multidrug resistance (MDR) IncC plasmid (pPm14C18) recovered from Proteus mirabilis. pPm14C18 was identified in a CMY-2-producing P. mirabilis isolate from chicken in China in 2014, and could be transferred to Escherichia coli conferring an MDR phenotype. Whole genome sequencing confirmed pPm14C18 was a novel type 1/2 hybrid IncC plasmid 165,992bp in size, containing fifteen antimicrobial resistance genes. It harboured a novel MDR mosaic region comprised of a hybrid Tn21-pDU, in which bla, dfrA32 and ereA were firstly reported in IncC plasmid. Phylogenetic relationship reconstruction based on the nucleotide sequences of the 52 IncC backbones showed all type 1 IncC plasmids were clustered into one clade, and then merged with pPm14C18 and finally with the type 2 IncC plasmids and another type 1/2 hybrid IncC plasmid pYR1. The MDR IncC plasmids in P. mirabilis of animal origin might threaten public health, which should be drawn more attention.
IncC质粒作为广谱头孢菌素和碳青霉烯类耐药基因bla和bla的载体备受关注。本研究的目的是对一株从奇异变形杆菌中分离得到的多重耐药（MDR）IncC质粒（pPm14C18）进行测序和特征分析。pPm14C18于2014年在中国一株产CMY-2的鸡源奇异变形杆菌分离株中被鉴定出来，并且能够转移至大肠杆菌并赋予其多重耐药表型。全基因组测序证实pPm14C18是一种新型的1/2型杂交IncC质粒，大小为165,992bp，含有15个抗菌药物耐药基因。它含有一个由杂交Tn21-pDU组成的新型多重耐药镶嵌区域，其中bla、dfrA32和ereA首次在IncC质粒中被报道。基于52个IncC主干核苷酸序列重建的系统发育关系表明，所有1型IncC质粒聚为一个分支，然后与pPm14C18合并，最终与2型IncC质粒以及另一个1/2型杂交IncC质粒pYR1合并。动物源奇异变形杆菌中的多重耐药IncC质粒可能会威胁公共卫生，应予以更多关注。
