miR-34a 和 FoxO1 通过 Erk 信号通路调控 PDGFRα 促进猪肌肉前体脂肪细胞成脂分化。

PDGFRα Regulated by miR-34a and FoxO1 Promotes Adipogenesis in Porcine Intramuscular Preadipocytes through Erk Signaling Pathway.

机构信息

Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.

出版信息

Int J Mol Sci. 2017 Nov 15;18(11):2424. doi: 10.3390/ijms18112424.

Suitable intramuscular fat (IMF) content improves porcine meat quality. The vital genes regulating IMF deposition are necessary for the selection and breeding of an IMF trait. However, the effect and mechanism of on IMF deposition are still unclear. Here, is moderately expressed in porcine longissimus dorsi muscle (LD), whereas it highly expressed in white adipose tissue (WAT). Moreover, -positive cells were located in the gaps of LD fibers which there were IMF adipocytes. Compared with 180-day-old and lean-type pigs, the levels of were much higher in one-day-old and fat-type pigs. Meanwhile the levels of gradually decreased during IMF preadipocyte differentiation. Furthermore, promoted adipogenic differentiation through activating Erk signaling pathway. Based on upstream regulation analysis, we found that the knockdown of repressed lipogenesis by downregulating , and miR-34a inhibited adipogenesis through targeting . Collectively, is a positive regulator of IMF deposition. Therefore, we suggest that is a possible target to improve meat quality.

合适的肌内脂肪（IMF）含量可以改善猪肉品质。调节 IMF 沉积的关键基因是 IMF 特性选择和培育所必需的。然而，对 IMF 沉积的影响和机制尚不清楚。在这里，在猪背最长肌（LD）中适度表达，而在白色脂肪组织（WAT）中高度表达。此外，-阳性细胞位于 LD 纤维的间隙中，那里有 IMF 脂肪细胞。与 180 日龄和瘦肉型猪相比，1 日龄和脂肪型猪的水平要高得多。同时，随着 IMF 前体脂肪细胞分化，水平逐渐降低。此外，通过激活 Erk 信号通路促进脂肪生成分化。基于对的上游调控分析，我们发现敲低通过下调抑制脂肪生成，miR-34a 通过靶向抑制脂肪生成。总之，是 IMF 沉积的正调控因子。因此，我们认为是改善肉质的一个潜在靶点。