如何拆分用于DNA检测的G-四链体：对DNA拆分G-四链体形成的新见解。
How to split a G-quadruplex for DNA detection: new insight into the formation of DNA split G-quadruplex.
作者信息
Zhu Jinbo, Zhang Libing, Dong Shaojun, Wang Erkang
机构信息
State Key Laboratory of Electroanalytical Chemistry , Changchun Institute of Applied Chemistry , Chinese Academy of Sciences , Changchun 130022 , P. R. China . Email:
University of Chinese Academy of Sciences , Beijing , 100049 , P. R. China.
出版信息
Chem Sci. 2015 Aug 1;6(8):4822-4827. doi: 10.1039/c5sc01287b. Epub 2015 Jun 2.
Here, we get a new insight into the formation of a split G-quadruplex from the viewpoints of the split mode and guanine base number. An unusual result is that the split mode 4 : 8 performed best in six split modes, including the frequently used mode 1 : 3 and 2 : 2 in the split G-quadruplex enhanced fluorescence assay. Circular dichroism spectra verified the conclusion. The application of the split G-quadruplex based assay in DNA detection was performed on the point mutations of the JAK2 V617F and HBB genes. A multi-target analysis method based on a pool of G-segments split from T30695 (GGGTGGGTGGGTGGGT) by the magic "law of 4 : 8" was established.
在此，我们从分裂模式和鸟嘌呤碱基数目的角度，对分裂型G-四链体的形成有了新的认识。一个不同寻常的结果是，在六种分裂模式中，包括在分裂型G-四链体增强荧光测定中常用的1 : 3和2 : 2模式，分裂模式4 : 8表现最佳。圆二色光谱证实了这一结论。基于分裂型G-四链体的检测方法在JAK2 V617F和HBB基因的点突变DNA检测中得到了应用。建立了一种基于通过神奇的“4 : 8法则”从T30695（GGGTGGGTGGGTGGGT）分裂得到的一组G片段的多靶点分析方法。
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