Lim Myung-Jin, Jang Hyun-Jin, Yu Mi-Kyung, Lee Kwang-Won, Min Kyung-San
Department of Conservative Dentistry, Chonbuk National University School of Dentistry and Institute of Oral Bioscience, Jeonju, Korea.
Department of Life Science, College of Natural Sciences, Chonbuk National University, Jeonju, Korea.
Restor Dent Endod. 2017 Nov;42(4):290-300. doi: 10.5395/rde.2017.42.4.290. Epub 2017 Oct 20.
This study investigated the removal efficacy and cytotoxicity of a newly developed calcium hydroxide paste (cleaniCal, Maruchi) using -2-methyl-pyrrolidone (NMP) as a vehicle in comparison with ApexCal (Ivoclar Vivadent) and Calcipex II (Nishika), which use different vehicles such as polyethylene glycol and propylene glycol, respectively.
Thirty maxillary premolars with oval-shaped canals were divided into 3 groups and the teeth were filled with one of the pastes. After removal of the paste, micro-computed tomographic (μ-CT) imaging was obtained to assess the volume of residual paste in the root canal of each tooth. The teeth were then split longitudinally and the area of the paste-coated surface was evaluated by stereomicroscopy. The cytotoxicity of each product was assessed using an agar overlay assay. The effect of each vehicle on cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The data were analyzed using one-way analysis of variance and Tukey's tests to detect any significance ( < 0.05).
In the μ-CT and stereomicroscopic analysis, cleaniCal exhibited less remnants of medicament than ApexCal and Calcipex. cleaniCal showed a higher cytotoxicity than the other pastes in the agar overlay assay. Furthermore, NMP exhibited lower cell viability compared to the other vehicles.
cleaniCal showed better removal efficacy compared to the other products. However, clinicians should be aware of the higher cytotoxicity of the NMP-based material and consider its possible adverse effects on periradicular tissue when it is overfilled.
本研究调查了一种新开发的以2-甲基吡咯烷酮（NMP）为载体的氢氧化钙糊剂（cleaniCal，Maruchi）的去除效果和细胞毒性，并与分别使用聚乙二醇和丙二醇等不同载体的ApexCal（Ivoclar Vivadent）和Calcipex II（Nishika）进行比较。
将30颗具有椭圆形根管的上颌前磨牙分为3组，并用其中一种糊剂充填。去除糊剂后，进行微计算机断层扫描（μ-CT）成像，以评估每颗牙齿根管内残留糊剂的体积。然后将牙齿纵向劈开，通过体视显微镜评估糊剂涂层表面的面积。使用琼脂覆盖试验评估每种产品的细胞毒性。使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）试验评估每种载体对细胞活力的影响。使用单因素方差分析和Tukey检验分析数据，以检测任何显著性差异（P<0.05）。
在μ-CT和体视显微镜分析中，cleaniCal显示出比ApexCal和Calcipex更少的药物残留。在琼脂覆盖试验中，cleaniCal显示出比其他糊剂更高的细胞毒性。此外，与其他载体相比，NMP表现出较低的细胞活力。
与其他产品相比，cleaniCal显示出更好的去除效果。然而，临床医生应注意基于NMP的材料具有更高的细胞毒性，并在超充时考虑其对根尖周组织可能产生的不良反应。
