Kim Mi-Ah, Rosa Vinicius, Neelakantan Prasanna, Hwang Yun-Chan, Min Kyung-San
Department of Conservative Dentistry, School of Dentistry and Institute of Oral Bioscience, Jeonbuk National University, Jeonju 54896, Korea.
Discipline of Oral Sciences, Faculty of Dentistry, National University of Singapore, Singapore 119085, Singapore.
Materials (Basel). 2021 May 27;14(11):2863. doi: 10.3390/ma14112863.
This study aimed to evaluate a newly developed pozzolan-based bioceramic sealer (PZBS) regarding setting time, radiopacity, antibacterial effect, and cytocompatibility. The PZBS was manufactured by mixing calcium hydroxide and silica. The pozzolan reaction was verified by identification of calcium silicate hydrate (C-S-H) using X-ray diffraction analysis. The initial setting time and radiopacity were measured using the ISO 6876/2012 protocol in comparison with other commercially available calcium silicate (CS) sealers. The antibacterial effect of PZBS on biofilms cultured in the bovine root canal was evaluated by measurement of colony-forming units and volume of biofilms in comparison with other calcium hydroxide pastes. The morphological features of the biofilms were observed by scanning electron microscopy (SEM). The cytocompatibility of PZBS was assessed by the viability of bone marrow-derived mesenchymal stem cells and scratch wound healing rate in comparison with other CS sealers. The morphology of the cells cultured on the tested sealers was observed by SEM. The detection of the CS peak confirmed the formation of C-S-H. The initial setting time of PZBS was around 11 h, which was twice as long as the other tested sealers. The radiopacity of PZBS was 4.3 mm/Al, which satisfied the ISO criteria. The antibacterial effect and cytocompatibility of PZBS were comparable to those of the commercially available intracanal medicaments and CS endodontic sealers, respectively. The PZBS has the potential to be used for root canal obturation, and is expected to exert a favorable antibacterial effect.
本研究旨在评估一种新开发的基于火山灰的生物陶瓷封闭剂（PZBS）的凝固时间、射线不透性、抗菌效果和细胞相容性。PZBS通过将氢氧化钙和二氧化硅混合制成。通过使用X射线衍射分析鉴定硅酸钙水合物（C-S-H）来验证火山灰反应。与其他市售硅酸钙（CS）封闭剂相比，按照ISO 6876/2012标准测量PZBS的初始凝固时间和射线不透性。与其他氢氧化钙糊剂相比，通过测量生物膜的菌落形成单位和生物膜体积来评估PZBS对牛根管内培养生物膜的抗菌效果。通过扫描电子显微镜（SEM）观察生物膜的形态特征。与其他CS封闭剂相比，通过骨髓间充质干细胞的活力和划痕伤口愈合率评估PZBS的细胞相容性。通过SEM观察在测试封闭剂上培养的细胞形态。CS峰的检测证实了C-S-H的形成。PZBS的初始凝固时间约为11小时，是其他测试封闭剂的两倍。PZBS的射线不透性为4.3 mm/Al，符合ISO标准。PZBS的抗菌效果和细胞相容性分别与市售根管内药物和CS牙髓封闭剂相当。PZBS有潜力用于根管充填，并有望发挥良好的抗菌作用。
