Quantitative RedOx Sensing Team, Department of Basic Medical Sciences for Radiation Damages, National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba-shi, Japan.
Department of Frontier Science for Imaging, School of Medicine, Gifu University, Gifu, Japan.
Magn Reson Med. 2018 Feb;79(2):1212-1218. doi: 10.1002/mrm.27008. Epub 2017 Nov 16.
Pharmacokinetics of the tri[8-carboxy-2,2,6,6-tetrakis(2-hydroxymethyl)benzo[1,2-d:4,5-d']bis(1,3)dithio-4-yl]methyl radical (Oxo63) after a single bolus and/or continuous intravenous infusion was investigated in tumor-bearing C3H mice with or without body temperature control while under anesthesia.
The in vivo time course of Oxo63 in blood was measured using X-band electron paramagnetic resonance spectroscopy. Distribution of Oxo63 in normal muscle and tumor tissues was obtained using a surface coil resonator and a 700-MHz electron paramagnetic resonance spectrometer. The whole-body distribution of Oxo63 was obtained by 300-MHz continuous-wave electron paramagnetic resonance imaging. The high-resolution 300-MHz time-domain electron paramagnetic resonance imaging was also carried out to probe the distribution of Oxo63.
Urination of mice was retarded at low body temperature, causing the concentration of Oxo63 in blood to attain high levels. However, the concentration of Oxo63 in tumor tissue was lower with no control of body temperature than active body temperature control. The nonsystemized blood flow in the tumor tissues may pool Oxo63 at lower body temperature.
Pharmacokinetics of the contrast agent were found to be significantly affected by body temperature of the experimental animal, and can influence the probe distribution and the image patterns. Magn Reson Med 79:1212-1218, 2018. © Published 2017. This article is a U.S. Government work and is in the public domain in the USA.
在麻醉状态下，伴有或不伴有体温控制的荷瘤 C3H 小鼠中，研究了三[8-羧基-2,2,6,6-四（2-羟甲基）苯并[1,2-d:4,5-d']双（1,3）二硫-4-基）甲基]自由基（Oxo63）单次推注和/或连续静脉输注后的药代动力学。
使用 X 波段电子顺磁共振波谱法测量血液中 Oxo63 的体内时间过程。使用表面线圈谐振器和 700MHz 电子顺磁共振波谱仪获得正常肌肉和肿瘤组织中 Oxo63 的分布。通过 300MHz 连续波电子顺磁共振成像获得全身 Oxo63 分布。还进行了高分辨率 300MHz 时域电子顺磁共振成像以探测 Oxo63 的分布。
体温较低时，小鼠排尿延迟，导致血液中 Oxo63 浓度达到较高水平。然而，与主动体温控制相比，不控制体温时肿瘤组织中 Oxo63 的浓度较低。肿瘤组织中无系统血流可能会使 Oxo63 在较低体温下积聚。
实验动物的体温对造影剂的药代动力学有显著影响，并可能影响探针分布和图像模式。磁共振医学 79:1212-1218，2018。© 2017 年出版。本文是美国政府的工作，在美国公共领域。
