Institute for Integrative Systems Biology (I(2)SysBio), Paterna, Valencia, Spain; Robert Boyle Institut e.V., Jena, Germany.
Leibniz Institute for Agricultural Engineering and Bioeconomy (ATB), Bioengineering, Potsdam, Germany.
Bioresour Technol. 2018 Feb;249:1074-1079. doi: 10.1016/j.biortech.2017.10.093. Epub 2017 Oct 31.
We report here the impact of heat-shock treatments (55 and 70 °C) on the biogas production within the acidification stage of a two-stage reactor system for anaerobic digestion and biomethanation of grass. The microbiome proved both taxonomically and functionally very robust, since heat shocks caused minor community shifts compared to the controls, and biogas yield was not decreased. The strongest impact on the microbial profile was observed with a combination of heat shock and low pH. Since no transient reduction of microbial diversity occured after the shock, biogas keyplayers, but also potential pathogens, survived the treatment. All along the experiment, the heat-resistant bacterial profile consisted mainly of Firmicutes, Bacteroidetes and Proteobacteria. Bacteroides and Acholeplasma were reduced after heat shocks. An increase was observed for Aminobacterium. Our results prove the stability to thermal stresses of the microbial communities involved in acidification, and the resilience in biogas production irrespectively of the thermal treatment.
我们在此报告热休克处理（55 和 70°C）对草的厌氧消化和生物甲烷化两级反应系统酸化阶段生物沼气产生的影响。微生物组在分类和功能上都非常稳健，因为与对照相比，热冲击引起的群落变化较小，生物沼气产量没有下降。与对照相比，热冲击和低 pH 值的组合对微生物分布的影响最大。由于冲击后微生物多样性没有暂时减少，生物沼气的关键参与者，甚至潜在的病原体，都能在处理中存活下来。在整个实验过程中，耐热细菌主要由厚壁菌门、拟杆菌门和变形菌门组成。热冲击后，拟杆菌属和无壁菌属减少，而氨单胞菌属增加。我们的结果证明了参与酸化的微生物群落对热应激的稳定性，以及在不受热处理影响的情况下生物沼气生产的弹性。
