Guangzhou Quality Supervision and Testing Institute, Guangzhou 511447, China; Guangzhou City Key Laboratory of Detection Technology for Food Safety of, Guangzhou 511447, China; Guangzhou City Research Center of Risk Dynamic Detection and Early Warning for Food Safety, Guangzhou 511447, China.
Guangzhou Quality Supervision and Testing Institute, Guangzhou 511447, China; Guangzhou City Key Laboratory of Detection Technology for Food Safety of, Guangzhou 511447, China; Guangzhou City Research Center of Risk Dynamic Detection and Early Warning for Food Safety, Guangzhou 511447, China.
Food Chem. 2018 Mar 15;243:162-167. doi: 10.1016/j.foodchem.2017.09.110. Epub 2017 Sep 25.
A sensitive UPLC-MS/MS method was established for the simultaneous determination of seven fluorescent whitening agents in flour matrix. Samples were ultrasonically extracted by trichloromethane-acetone. Then analytes were separated on a C18 column and detected by MS/MS. The developed method was validated in terms of the linearity, matrix effect, accuracy and precision. The method showed a good linearity (r>0.997) for all analytes in their respective concentration ranges. The LOQ for seven analytes were in the range of 2.5-25.0μg/kg. The matrix effect was not significant and almost negligible. The average recoveries of seven analytes from the negative samples spiked at three different concentrations were in a range from 81.5% to 105%. The intra-day precision and inter-day precision were in the ranges of 2.9-9.0% and 6.7-11.2%. The developed method was successfully applied to analyze forty commercial flours and FWA184 was detected in three samples with the concentrations of 31-60µg/kg.
建立了一种灵敏的 UPLC-MS/MS 方法，用于同时测定面粉基质中的七种荧光增白剂。样品用三氯甲烷-丙酮超声提取。然后，用 C18 柱分离分析物，并通过 MS/MS 检测。该方法在线性、基质效应、准确度和精密度方面进行了验证。该方法在各自的浓度范围内对所有分析物均表现出良好的线性（r>0.997）。七种分析物的 LOQ 在 2.5-25.0μg/kg 范围内。基质效应不显著，几乎可以忽略不计。七种分析物从三个不同浓度添加的阴性样品中的平均回收率在 81.5%-105%之间。日内精密度和日间精密度的范围分别为 2.9%-9.0%和 6.7%-11.2%。该方法成功应用于分析四十种商业面粉，并在三种样品中检测到 FWA184，浓度为 31-60μg/kg。
