Wang Tianjiao, Wu Pinggu, Hu Zhengyan, Wang Liyuan, Tang Jun, Jiang Wei, Wang Zhiyuan
Department of Public Health of Zhejiang University, Hangzhou 310058, China.
Wei Sheng Yan Jiu. 2016 Jul;45(4):663-667.
To establish a new qualitative and quantitative ultraperformance liquid chromatography-fluorescence detector / photodiode array detector with series double-detector method for the determination of eleven fluorescent whitening agents in paper food packaging materials.
The sample was extracted with 40%acetonitrile water solution, separated by Waters ACQUITY UPLC BEH C_(18)column( 1. 7μm, 2. 1 mm × 100 mm) and eluted gradient. The excitation wavelength and emission wavelength of fluorescence detector( FLD) were 350 nm and 430 nm, and the wavelength of photodiode array detector( PDA) was 350 nm. The detectors were used in series to achieve qualitative and quantitative detection.
In the substrates of paper cups, paper bowls, paper trays and paper boxes, those eleven fluorescent whitening agents were separated properly. For both detectors, in the linear range of 25- 1000 ng / m L, the correlation coefficient was greater than 0. 99, and the recoveries of spiked recoveries were between 82. 2%- 104. 1% with the RSD less than 10%( n = 6). The detection limits ofthose eleven fluorescent whitening agents were 0. 20- 0. 28 mg / kg for FLD and 1. 4- 2. 5mg / kg for PDA.
The eleven fluorescent whitening agents could be separated properly with complete separation, good shapes and high recovery rate. This method is easy to operate also. Thus it's an effective method to detect the fluorescent whitening agents in paper food packaging materials.
建立一种新型的超高效液相色谱-荧光检测器/光电二极管阵列检测器串联双检测器法，用于测定纸质食品包装材料中的11种荧光增白剂。
样品用40%乙腈水溶液提取，采用Waters ACQUITY UPLC BEH C₁₈柱（1.7μm，2.1mm×100mm）分离并梯度洗脱。荧光检测器（FLD）的激发波长和发射波长分别为350nm和430nm，光电二极管阵列检测器（PDA）的波长为350nm。两个检测器串联使用以实现定性和定量检测。
在纸杯、纸碗、纸托盘和纸盒的基质中，这11种荧光增白剂得到了良好的分离。对于两种检测器，在25 - 1000ng/mL的线性范围内，相关系数大于0.99，加标回收率在82.2% - 104.1%之间，相对标准偏差小于10%（n = 6）。这11种荧光增白剂的检测限，荧光检测器为0.20 - 0.28mg/kg，光电二极管阵列检测器为1.4 - 2.5mg/kg。
该方法能将11种荧光增白剂完全分离，峰形良好，回收率高，操作简便，是检测纸质食品包装材料中荧光增白剂的有效方法。
