Department of Chemistry and NIS Interdepartmental Centre, University of Torino, via Pietro Giuria 7, 10125 Torino, Italy.
Molecular Biotechnology and Health Sciences Department, University of Torino, via Quarello 15, 10135 Torino, Italy.
Int J Pharm. 2018 Jan 15;535(1-2):438-443. doi: 10.1016/j.ijpharm.2017.11.026. Epub 2017 Nov 13.
The interaction between two mucin types (mucin from porcine stomach - PGM and mucin from bovine submaxillary glands - BSM) and gold nanoparticles (GNPs) of various size (5, 20 and 40 nm) and functionalization (with cysteamine or thioglycolic acid) was studied under physiological conditions, in order to investigate the affinity of the nanoparticles to the proteins. Different methods are employed to monitor the interactions: UV-vis and fluorescence spectroscopy, fluorescence lifetime, circular dichroism and transmission electron microscopy. These studies have shown the formation of a complex between GNPs and both PGM and BSM. This aspect could be of great importance for the use of gold nanoparticles for biomedical purposes in those diseases where qualitative and quantitative mucin anomalies play an essential role in mucus composition and rheology.
研究了两种黏蛋白类型（猪胃黏蛋白 - PGM 和牛颌下腺黏蛋白 - BSM）与不同大小（5、20 和 40nm）和功能化（半胱胺或巯基乙酸）的金纳米粒子（GNPs）在生理条件下的相互作用，以研究纳米粒子与蛋白质的亲和力。采用不同的方法监测相互作用：紫外-可见和荧光光谱、荧光寿命、圆二色性和透射电子显微镜。这些研究表明，GNPs 与 PGM 和 BSM 之间形成了复合物。这一方面对于将金纳米粒子用于那些定性和定量黏蛋白异常在黏液组成和流变学中起重要作用的疾病中的生物医学目的可能非常重要。
