Wang Yue-Qiang, Liu Xiao-Ming, Tang Jia, Yang Gui-Qin, Yu Zhen
Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Guangdong Institute of Eco-environmental Science and Technology, Guangzhou 510650, PR China.
Int J Syst Evol Microbiol. 2018 Jan;68(1):113-117. doi: 10.1099/ijsem.0.002467. Epub 2017 Nov 17.
A novel halophilic bacterium, strain GSS13, capable of growing at salinities of 8-28 % (w/v) NaCl (optimally at 24 %, w/v) was isolated from Yuncheng Saline Lake in China. GSS13 was Gram-stain-positive, strictly aerobic, rod-shaped, motile and a non-spore-former. Growth occurred at pH 5.5-8.5 (optimum pH 7.0) and at 10-45 °C (optimum 30 °C). On the basis of the results of 16S rRNA gene sequences phylogenetic analyses, GSS13 represents a member of the genus Salibacterium and is closely related to Salibacterium halotolerans S7, Salibacterium qingdaonense CM1 and Salibacterium halochares MSS4, with 16S rRNA gene sequence similarities of 98.7, 98.4 and 97.9 %, respectively. The results of DNA-DNA pairing studies revealed that GSS13 displayed 52, 43 and 48 % relatedness to S. halotolerans S7, S. qingdaonense CM1 and S. halochares MSS4, respectively. The polar lipids of GSS13 consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol unidentified glycolipids, an unidentified phospholipid and an unidentified lipid. The predominant isoprenoid quinone was MK-7, and the major fatty acids were anteiso-C17 : 0 (32.0 %) and anteiso C15 : 0 (26.4 %). The DNA G+C content of the type strain was 52.1 mol%. On the basis of phylogenetic, chemotaxonomic and phenotypic data, a novel species of the genus Salibacterium is proposed, with the name Salibacterium lacus sp. nov. The type strain is GSS13 (=KCTC 33792=MCCC 1K00567).
从中国运城盐湖中分离出一株新型嗜盐细菌，菌株GSS13，其能够在8 - 28%（w/v）NaCl盐度下生长（最适盐度为24%，w/v）。GSS13革兰氏染色阳性，严格需氧，杆状，具运动性，不产芽孢。在pH 5.5 - 8.5（最适pH 7.0）和10 - 45℃（最适30℃）条件下生长。基于16S rRNA基因序列系统发育分析结果，GSS13属于盐杆菌属成员，与耐盐盐杆菌S7、青岛盐杆菌CM1和盐沼盐杆菌MSS4密切相关，16S rRNA基因序列相似性分别为98.7%、98.4%和97.9%。DNA - DNA杂交研究结果表明，GSS13与耐盐盐杆菌S7,、青岛盐杆菌CM1和盐沼盐杆菌MSS4的亲缘关系分别为52%、43%和48%。GSS13的极性脂质由双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、未鉴定的糖脂、一种未鉴定的磷脂和一种未鉴定的脂质组成。主要类异戊二烯醌为MK - 7，主要脂肪酸为anteiso - C17 : 0（32.0%）和anteiso C15 : 0（26.4%）。模式菌株的DNA G + C含量为52.1 mol%。基于系统发育、化学分类和表型数据，提出盐杆菌属一个新物种，命名为盐湖盐杆菌（Salibacterium lacus sp. nov.）。模式菌株为GSS13（=KCTC 33792 = MCCC 1K00567）。
