Singh Harjodh, Kaur Manpreet, Singh Shweta, Mishra Sunita, Kumar Shekhar, Vemuluri Venkata Ramana, Tanuku Naga Radha Srinivas, Pinnaka Anil Kumar
1Academy of Scientific and Innovative Research, (AcSIR), CSIR Campus, Chennai, India.
2MTCC-Microbial Type Culture Collection and Gene Bank, CSIR-Institute of Microbial Technology, Chandigarh-160036, India.
Int J Syst Evol Microbiol. 2018 Nov;68(11):3506-3511. doi: 10.1099/ijsem.0.003021. Epub 2018 Sep 18.
A strictly aerobic, haloalkali-tolerant, Gram-stain-positive, non-motile, rod-shaped bacterium, designated strain SMB4, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India. Growth occurred at 25-50 °C, 4-12 % (w/v) NaCl and pH of 5-9. Strain SMB4 was positive for β-galactosidase, oxidase, catalase and urease activities. The fatty acids were dominated by branched forms of fatty acids with iso- and anteiso-saturated fatty acids, with a high abundance of anteiso-C15 : 0, anteiso-C17 : 0 and C18 : 0. The cell-wall peptidoglycan of strain SMB4 contained meso-diaminopimelic acid, while the polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and three unidentified lipids. The DNA G+C content of strain SMB4 was 49.1 mol%. A blast sequence similarity search based on 16S rRNA gene sequence indicated that Salibacterium halochares, Salibacterium halotolerans and Salibacterium qingdaonense were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 98.4, 98.2 and 97.0 % respectively. Phylogenetic analysis showed that strain SMB4 was clustered with S. halochares and together clustered with S. halotolerans and S. qingdaonense. DNA-DNA hybridization of strain SMB4 with S. halochares DSM 21373, S. halotolerans S7 and S. quigdaonense DSM 21621 showed a relatedness values of only 39.8, 26.3 and 42.8 %, respectively. Based on its phenotypic characteristics and on phylogenetic inference, strain SMB4 represents a novel species of the genus Salibacterium, for which the name Salibacterium nitratireducens sp. nov. is proposed. The type strain is SMB4 (=MTCC 12633=KCTC 33876=JCM 32187).
从印度拉贾斯坦邦桑伯尔盐湖采集的水样中分离出一株严格需氧、耐盐碱、革兰氏染色阳性、无运动性的杆状细菌，命名为SMB4菌株。该菌株在25-50 °C、4-12%（w/v）NaCl和pH值为5-9的条件下生长。SMB4菌株的β-半乳糖苷酶、氧化酶、过氧化氢酶和脲酶活性呈阳性。脂肪酸以异饱和脂肪酸和anteiso饱和脂肪酸的支链形式为主，anteiso-C15 : 0、anteiso-C17 : 0和C18 : 0含量较高。SMB4菌株的细胞壁肽聚糖含有内消旋二氨基庚二酸，而极性脂质包括双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的磷脂和三种未鉴定的脂质。SMB4菌株的DNA G+C含量为49.1 mol%。基于16S rRNA基因序列的blast序列相似性搜索表明，盐栖盐杆菌、耐盐盐杆菌和青岛盐杆菌是最近的系统发育邻居，两两序列相似性分别为98.4%、98.2%和97.0%。系统发育分析表明，SMB4菌株与盐栖盐杆菌聚类，并与耐盐盐杆菌和青岛盐杆菌一起聚类。SMB4菌株与盐栖盐杆菌DSM 21373、耐盐盐杆菌S7和青岛盐杆菌DSM 21621的DNA-DNA杂交显示，相关性值分别仅为39.8%、26.3%和42.8%。基于其表型特征和系统发育推断，SMB4菌株代表盐杆菌属的一个新物种，为此提出新名称硝酸盐还原盐杆菌（Salibacterium nitratireducens sp. nov.）。模式菌株为SMB4（=MTCC 12633=KCTC 33876=JCM 32187）。
