Shi Wen, Li Xiaohua, Ma Huimin
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.
Methods Appl Fluoresc. 2014 Dec 12;2(4):042001. doi: 10.1088/2050-6120/2/4/042001.
Intracellular pH regulates a number of cell metabolism processes and its sensing is thus of great importance for cell studies. Among various methods, fluorescent probes have been widely used for sensing intracellular pH values because of their high sensitivity and spatiotemporal resolution capability. In this article, the development of fluorescent probes with good practicability in sensing intracellular pH values and pH variation during 2009 - 2014 is reviewed. These fluorescence probes are divided into two kinds: small molecules and nanoparticles. Photophysical properties, advantages/disadvantages and applications of the two kinds of probes are discussed in detail.
细胞内pH值调节许多细胞代谢过程，因此其传感对于细胞研究非常重要。在各种方法中，荧光探针因其高灵敏度和时空分辨率能力而被广泛用于检测细胞内pH值。本文综述了2009年至2014年间在检测细胞内pH值和pH变化方面具有良好实用性的荧光探针的发展情况。这些荧光探针分为两类：小分子和纳米颗粒。详细讨论了这两类探针的光物理性质、优缺点及应用。
