Kyrychenko Alexander
Institute of Chemistry and School of Chemistry, V. N. Karazin Kharkiv National University, 4 Svobody Square, Kharkiv 61022, Ukraine.
Methods Appl Fluoresc. 2015 Oct 1;3(4):042003. doi: 10.1088/2050-6120/3/4/042003.
Fluorescence techniques have become powerful and widely used tools for studies of biochemical and biophysical processes occurring in biological membranes. Various fluorescence methods have played and continue to play key roles in modern membrane science, so that there have been several focused reviews on this topic. Here, I present the progress and recent achievements in various fluorescence approaches commonly utilized in studies of biological membranes. Applications of numerous fluorescence methods have been reviewed, including single molecule detection, confocal scanning fluorescence microscopy and fluorescence lifetime imaging. I focus on the benefits and limitations of various fluorescence techniques and their combinations, as well as the available methods of in vivo studying. A separate section is dedicated to discussing and comparing different classes of fluorescent membrane probes and their applications to the study of biological membranes. The review should provide researchers from chemistry, biochemistry, and biophysics with the necessary background to identify a range of suitable fluorescence methods in order to successfully design and conduct experimental studies on model lipid bilayers and biological membranes.
荧光技术已成为研究生物膜中发生的生化和生物物理过程的强大且广泛使用的工具。各种荧光方法在现代膜科学中已经发挥并将继续发挥关键作用，因此已有几篇关于该主题的重点综述。在此，我介绍了生物膜研究中常用的各种荧光方法的进展和最新成果。已对众多荧光方法的应用进行了综述，包括单分子检测、共聚焦扫描荧光显微镜和荧光寿命成像。我重点关注各种荧光技术及其组合的优点和局限性，以及体内研究的可用方法。单独的一节专门讨论和比较不同类别的荧光膜探针及其在生物膜研究中的应用。这篇综述应为化学、生物化学和生物物理学领域的研究人员提供必要的背景知识，以便他们确定一系列合适的荧光方法，从而成功地设计和开展关于模型脂质双层和生物膜的实验研究。
