Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Jiangsu Academy of Agricultural Science, Nanjing, China.
Pest Manag Sci. 2018 Apr;74(4):950-958. doi: 10.1002/ps.4794. Epub 2018 Jan 5.
The beet armyworm Spodoptera exigua, a worldwide phytophagous pest, causes considerable economic agricultural losses. Understanding the relationship between its fecundity and the host plant is a basic and important component of early forecasting of beet armyworm outbreaks. However, little is known about the molecular mechanism by which distinct hosts affect S. exigua fecundity.
In this study, key life-history parameters of S. exigua reared on distinct hosts were investigated; the host plants could be ranked as lettuce > shallot > tomato > celery in their order of suitability. Full-length S. exigua vitellogenin receptor (SeVgR) cDNA was cloned, and sex-, stage- and tissue-specific expression characteristics were assessed. Spodoptera exigua vitellogenin (SeVg) and SeVgR expression levels were markedly modulated by host nutrients (P < 0.05). SeVg and SeVgR expression levels were significantly higher in S. exigua reared on lettuce, the most preferred and most nutritive host, than in those reared on tomato and celery. Interestingly, significant linear regression correlations were found between SeVg and SeVgR expression levels and key S. exigua life-history parameters, especially life span, pupa weight, and female fecundity (P < 0.01).
Host plant type and suitability could affect the expression pattern of SeVg and SeVgR, which influenced S. exigua fecundity. Vg and VgR have the potential to be used as molecular markers of S. exigua fecundity and for forecasting outbreaks of S. exigua on different hosts. © 2017 Society of Chemical Industry.
甜菜夜蛾 Spodoptera exigua 是一种世界性的植食性害虫，会对农业造成严重的经济损失。了解其繁殖力与宿主植物之间的关系是甜菜夜蛾暴发早期预测的基本和重要组成部分。然而，对于不同宿主如何影响甜菜夜蛾繁殖力的分子机制却知之甚少。
本研究调查了在不同宿主上饲养的甜菜夜蛾的关键生活史参数；按照适宜性的顺序，宿主植物可分为生菜> 青葱> 番茄> 芹菜。克隆了甜菜夜蛾卵黄原蛋白受体（SeVgR）全长 cDNA，并评估了其性别、阶段和组织特异性表达特征。宿主营养物质显著调节了甜菜夜蛾卵黄蛋白（SeVg）和 SeVgR 的表达水平（P < 0.05）。在以生菜为食的甜菜夜蛾中，SeVg 和 SeVgR 的表达水平明显高于以番茄和芹菜为食的甜菜夜蛾。有趣的是，在关键的甜菜夜蛾生活史参数（尤其是寿命、蛹重和雌虫繁殖力）与 SeVg 和 SeVgR 的表达水平之间发现了显著的线性回归相关性（P < 0.01）。
宿主植物类型和适宜性可能会影响 SeVg 和 SeVgR 的表达模式，从而影响甜菜夜蛾的繁殖力。Vg 和 VgR 有可能成为甜菜夜蛾繁殖力的分子标记物，用于预测不同宿主上甜菜夜蛾的暴发。 © 2017 化学工业学会。
