Gao Xuyuan, Tian Zhenya, Zhang Yan, Chen Guangmei, Ma Chao, Tian Zhenqi, Cui Shaowei, Lu Yongyue, Zhou Zhongshi
College of Agriculture, South China Agricultural University, Guangzhou, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Physiol. 2020 May 5;11:417. doi: 10.3389/fphys.2020.00417. eCollection 2020.
Increase in atmospheric CO directly affects the insect physiology and behavior, and indirectly affects the herbivorous insects by affecting their hosts. The increase in atmospheric CO is accompanied by an increase in temperature and heat waves. LeSage is a natural enemy of (common ragweed). The development and reproduction of this beetle is weakened upon eating common ragweed grown under stress conditions. As female behavior and physiology alter after mating, the reproductive tract of males is likely to modulate reproduction and development in this species. Herein, the transcriptional profiles of testes and accessory glands from male individuals feeding on common ragweed under conditions of high CO concentration and heat waves and that grown under ambient CO concentration were compared. Differentially expressed genes (DEGs) were identified between the same tissues from beetles fed on common ragweed grown under different stress conditions. There were 3, 2, 3, 1and 5 genes related to decomposition and transport of macromolecular substances, host location, stress response, reproduction, and poisonous food-utilization. No expected response was observed in the male reproductive tract, but some of the identified DEGs might control the development of the population. The results presented here should be helpful in guiding future studies on deciphering the indirect response of other organs to high CO concentration and heat waves, as well as the functions of seminal fluid proteins in .
大气中二氧化碳浓度升高直接影响昆虫的生理和行为，并通过影响其寄主间接影响植食性昆虫。大气中二氧化碳浓度升高伴随着气温升高和热浪增加。勒萨热甲虫是豚草的天敌。这种甲虫取食在胁迫条件下生长的豚草后，其发育和繁殖会受到削弱。由于雌虫在交配后行为和生理会发生改变，雄虫的生殖道可能会调节该物种的繁殖和发育。在此，比较了在高二氧化碳浓度和热浪条件下取食豚草的雄性个体以及在环境二氧化碳浓度下生长的雄性个体的睾丸和附腺的转录谱。在取食不同胁迫条件下生长的豚草的甲虫相同组织之间鉴定出了差异表达基因（DEG）。有3、2、3、1和5个基因分别与大分子物质的分解和运输、寄主定位、应激反应、繁殖以及有毒食物利用有关。在雄性生殖道中未观察到预期的反应，但一些已鉴定的差异表达基因可能控制种群的发育。本文给出的结果应有助于指导未来关于解读其他器官对高二氧化碳浓度和热浪的间接反应以及勒萨热甲虫精液蛋白功能的研究。
