Department of Plant Microbe Interactions, CSIR-NBRI, Lucknow, 226001, U.P, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, U.P, India.
Department of Plant Microbe Interactions, CSIR-NBRI, Lucknow, 226001, U.P, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, U.P, India.
Plant Physiol Biochem. 2017 Dec;121:216-225. doi: 10.1016/j.plaphy.2017.11.004. Epub 2017 Nov 10.
Tomato suffers a huge loss every year because of early blight disease. This study focuses on efficient inhibition of Alternaria solani, the causative agent of early blight disease in tomato in vitro and in vivo. Foliar spray of 5 μg/mL of biosynthesized silver nanoparticles in A. solani infected plants resulted in significant increase of 32.58% in fresh weight and 23.52% in total chlorophyll content of tomato as compared to A. solani infected plants. A decrease of 48.57, 30, 39.59 and 28.57% was observed in fungal spore count, lipid peroxidation, proline content and superoxide dismutase respectively in infected tomato plants after treatment with synthesized silver nanoparticles as compared to A. solani infected plants. No significant variation in terms of soil pH, cultured population, carbon source utilization pattern and soil enzymes including dehydrogenase, urease, protenase and β-glucosidase was observed after foliar spray of nanoparticles. It was revealed that direct killing of pathogens, increased photosynthetic efficiencies, increased plant resistance and decrease in stress parameters and stress enzymes are the mechanisms employed by plants and nanoparticles simultaneously to combat the biotic stress. Biosynthesized silver nanoparticles bear the potential to revolutionize plant disease management, though the molecular aspects of increased resistance must be looked upon.
番茄每年因早疫病损失巨大。本研究专注于高效抑制早疫病菌（导致番茄早疫病的病原体），在体外和体内均对其有抑制作用。叶面喷施 5μg/mL 的生物合成银纳米粒子可使感染早疫病菌的番茄植株鲜重增加 32.58%，总叶绿素含量增加 23.52%，与感染早疫病菌的番茄植株相比。与感染早疫病菌的番茄植株相比，处理后的番茄植株的真菌孢子数、脂质过氧化、脯氨酸含量和超氧化物歧化酶分别减少了 48.57%、30%、39.59%和 28.57%。叶面喷施纳米粒子后，土壤 pH 值、培养种群、碳源利用模式和土壤酶（包括脱氢酶、脲酶、蛋白酶和β-葡萄糖苷酶）均无显著变化。结果表明，病原体的直接杀灭、光合作用效率的提高、植物抗性的增强以及应激参数和应激酶的减少，是植物和纳米粒子协同抵抗生物胁迫的机制。生物合成银纳米粒子有可能彻底改变植物病害管理，尽管必须考虑增加抗性的分子方面。
