Liu Rong, Lv Xue, Wang Xiaohua, Yang Li, Cao Jun, Dai Ya, Wu Wang, Wu Yu
Faculty of Agriculture, Forestry and Food Engineering of Yibin University, Yibin, China.
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.
Front Plant Sci. 2023 Jun 5;14:1174450. doi: 10.3389/fpls.2023.1174450. eCollection 2023.
Wheat is one of the major food crops in the world. However, stripe rust fungus significantly decreases wheat yield and quality. In the present study, transcriptomic and metabolite analyses were conducted in R88 (resistant line) and CY12 (susceptible cultivar) during Pst-CYR34 infection due to the limited availability of information regarding the underlying mechanisms governing wheat-pathogen interactions. The results revealed that Pst infection promoted the genes and metabolites involved in phenylpropanoid biosynthesis. The key enzyme gene to regulate lignin and phenolic synthesis has a positive resistance contribution to Pst in wheat, which was verified by the virus-induced gene silencing (VIGS) technique. The distinctive resistance of R88 is regulated by the selective expression of genes involved in the fine-tuning of wheat-Pst interactions. Furthermore, metabolome analysis suggested that lignin biosynthesis-related metabolite accumulation was significantly affected by Pst. These results help to elucidate the regulatory networks of wheat-Pst interactions and pave the way for durable resistance breeding in wheat, which may ease environmental and food crises around the world.
小麦是世界主要粮食作物之一。然而，条锈菌会显著降低小麦的产量和品质。在本研究中，由于关于小麦与病原菌相互作用潜在机制的信息有限，因此在Pst-CYR34感染期间对R88（抗病品系）和CY12（感病品种）进行了转录组和代谢物分析。结果表明，Pst感染促进了参与苯丙烷生物合成的基因和代谢物。调控木质素和酚类合成的关键酶基因对小麦对Pst的抗性有积极贡献，这通过病毒诱导基因沉默（VIGS）技术得到了验证。R88独特的抗性是由参与微调小麦与Pst相互作用的基因的选择性表达调控的。此外，代谢组分析表明，Pst对木质素生物合成相关代谢物的积累有显著影响。这些结果有助于阐明小麦与Pst相互作用的调控网络，并为小麦的持久抗性育种铺平道路，这可能缓解全球的环境和粮食危机。
