Feng T, Schütz L F, Morrell B C, Perego M C, Spicer L J
Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.
Department of Animal Science, Oklahoma State University, Stillwater, OK, 74078, USA.
Anim Reprod Sci. 2018 Jan;188:85-92. doi: 10.1016/j.anireprosci.2017.11.012. Epub 2017 Nov 10.
Feeding N-carbamylglutamate (NCG) and arginine (ARG) improves reproductive measures in pigs and reduces systemic steroid levels in pregnant ewes. We hypothesized that the effects of NCG and ARG on reproduction were due to direct effects on the ovary. Thus, the objectives of this study were to investigate the effects of NCG and ARG on granulosa cell (GC) steroidogenesis, gene expression, and cell proliferation in vitro. GC were collected from small (1-5mm) bovine follicles and treated in vitro with NCG or ARG in serum-free medium for 24h to 48h. Both NCG and ARG inhibited (P<0.05) IGF1- and FSH-induced GC estradiol production but only NCG inhibited (P<0.05) progesterone production. In contrast, NCG and ARG increased (P<0.05) GC numbers induced by IGF1 and FSH. NCG inhibited (P<0.05) StAR, CYP11A1 and CYP19A1 mRNA abundance in small-follicle GC, whereas ARG had no effect (P>0.10) on StAR, CYP11A1 or CYP19A1 mRNA abundance. We conclude that NCG and ARG may act directly on GC and therefore may regulate ovarian function by slowing follicular differentiation via inhibiting IGF1 action, and steroid synthesis while stimulating GC proliferation in cattle.
饲喂N-氨甲酰谷氨酸（NCG）和精氨酸（ARG）可改善猪的繁殖性能，并降低怀孕母羊的全身类固醇水平。我们假设NCG和ARG对繁殖的影响是由于对卵巢的直接作用。因此，本研究的目的是调查NCG和ARG对体外颗粒细胞（GC）类固醇生成、基因表达和细胞增殖的影响。从直径1-5毫米的小型牛卵泡中收集颗粒细胞，并在无血清培养基中用NCG或ARG体外处理24至48小时。NCG和ARG均抑制（P<0.05）IGF1和FSH诱导的颗粒细胞雌二醇生成，但只有NCG抑制（P<0.05）孕酮生成。相反，NCG和ARG增加（P<0.05）IGF1和FSH诱导的颗粒细胞数量。NCG抑制（P<0.05）小卵泡颗粒细胞中StAR、CYP11A1和CYP19A1 mRNA丰度，而ARG对StAR、CYP11A1或CYP19A1 mRNA丰度无影响（P>0.10）。我们得出结论，NCG和ARG可能直接作用于颗粒细胞，因此可能通过抑制IGF1作用来减缓卵泡分化，同时抑制类固醇合成并刺激牛颗粒细胞增殖，从而调节卵巢功能。
