Institute of Animal Husbandry and Veterinary Medicine (IAHVM), Beijing Academy of Agriculture and Forestry Sciences (BAAFS), Beijing 100097, China; Joint Laboratory of Animal Science between IAHVM of BAAFS and Division of Agricultural Science and Natural Resource of Oklahoma State University, Beijing 100097, China.
Beijing General Station of Animal Husbandry, Beijing 100107, China.
Anim Reprod Sci. 2020 Oct;221:106569. doi: 10.1016/j.anireprosci.2020.106569. Epub 2020 Jul 30.
Supplementation of N-carbamylglutamate (NCG) improves gestation outcomes, with increased piglet within-litter uniformity of birth weight and reduced peripheral steroid concentrations in pregnant sows and ewes. It was hypothesized that the effect of NCG on placental function results from direct effects on the placental trophoblasts. There, therefore, was investigation of the effects of NCG on pig placental trophoblast (pTr) steroidogenesis, mRNA transcript abundance, and cell proliferation in vitro. The pTr were treated with NCG in serum-free medium for 24-48 h. Treatment with NCG inhibited pTr progesterone, androstenedione, testosterone (all P < 0.01), and estradiol (P < 0.05) production, whereas it promoted (P < 0.05) pTr proliferation. Treatment with NCG suppressed (P < 0.05) the relative abundances of CYP11A1, CYP19A1, and CASP3 and increased abundances of CCDN1 (P < 0.01) and CDK4 (P < 0.05) mRNA transcripts in pTr, whereas NCG treatment had no effect (P > 0.10) on relative abundances of StAR, HSD17B4, or HSD3B mRNA transcripts. Treatments with NCG can increase pTr cell numbers of sows through upregulating CCND1 and CDK4 and suppressing CASP3 mRNA transcript abundances, while modulating steroidogenesis through effects on CYP11A1 and CYP19A1 mRNA transcript abundances. It is concluded that NCG may have a direct action on pTr and may regulate placental function by suppressing pTr differentiation as a consequence of lesser steroid synthesis while promoting pTr proliferation and inhibiting apoptosis in sows.
补充 N- 氨甲酰谷氨酸（NCG）可改善妊娠结局，增加仔猪出生体重的同窝内均匀度，并降低妊娠母猪和母羊外周类固醇浓度。据推测，NCG 对胎盘功能的影响是由于其对胎盘滋养层的直接作用。因此，本研究调查了 NCG 对猪胎盘滋养层（pTr）类固醇生成、mRNA 转录丰度和体外细胞增殖的影响。将 pTr 在无血清培养基中用 NCG 处理 24-48 小时。NCG 处理抑制了 pTr 孕酮、雄烯二酮、睾酮（均 P<0.01）和雌二醇（P<0.05）的产生，而促进了 pTr 增殖（P<0.05）。NCG 处理抑制了 pTr 细胞中 CYP11A1、CYP19A1 和 CASP3 的相对丰度（P<0.05），并增加了 CCDN1（P<0.01）和 CDK4（P<0.05）mRNA 转录本的丰度，而 NCG 处理对 StAR、HSD17B4 或 HSD3B mRNA 转录本的相对丰度没有影响（P>0.10）。NCG 处理可通过上调 CCND1 和 CDK4 并抑制 CASP3 mRNA 转录本丰度，增加母猪的 pTr 细胞数量，同时通过影响 CYP11A1 和 CYP19A1 mRNA 转录本丰度来调节类固醇生成。因此，NCG 可能对 pTr 有直接作用，并通过抑制 pTr 分化来调节胎盘功能，因为类固醇合成减少，而促进 pTr 增殖并抑制母猪的凋亡。
