Lee Kyung Yeon, Moon Chang Hoon, Choi Seong Hoon
Department of Pediatrics, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan, Republic of Korea.
School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea.
Brain Dev. 2018 Mar;40(3):211-217. doi: 10.1016/j.braindev.2017.10.006. Epub 2017 Nov 14.
The purpose of this study was to identify whether there is an increase in type I interferon and proinflammatory cytokine levels in the cerebrospinal fluid of newborns with rotavirus-associated leukoencephalopathy.
Levels of type I interferons (interferon-alpha and interferon-beta) and proinflammatory cytokines (interleukin-6 and interferon-gamma) were measured in the cerebrospinal fluid of 23 newborns with rotavirus-associated leukoencephalopathy (patient group) and 39 infants under 90 days-of-age (control group).
Cerebrospinal fluid pleocytosis was not observed in either group. Cerebrospinal fluid interleukin-6 levels were significantly higher in the patient group (7.02 ± 5.88 pg/mL) than in the control group (1.14 ± 1.90 pg/mL) (p < .0001). The mean cerebrospinal fluid interferon-gamma levels of the patient group (24.43 ± 40.16 pg/mL) were also significantly higher than those of the controls group (0.0 ± 0.0 pg/mL) (p < .0001). Cerebrospinal fluid interferon-alpha was not detected in any patient (0%) from the patient group, but was detected in four (10.3%) of the controls. Interferon-beta was detected in only two patients (8.7%) from the patient group and in one (2.6%) of the controls. Cerebrospinal fluid interleukin-6 levels correlated positively with the extent of white matter lesions on diffusion-weighted magnetic resonance imaging (r = 0.607, p = .002).
Significant increases in proinflammatory cytokine levels accompanied by very low detection rates of type I interferon in cerebrospinal fluid indicate that rotavirus-associated leukoencephalopathy in newborns can be correlated with central nervous system inflammatory processes without direct virus invasion into the central nervous system.
本研究旨在确定轮状病毒相关性白质脑病新生儿脑脊液中I型干扰素和促炎细胞因子水平是否升高。
检测了23例轮状病毒相关性白质脑病新生儿（患者组）和39例90日龄以下婴儿（对照组）脑脊液中I型干扰素（α干扰素和β干扰素）和促炎细胞因子（白细胞介素-6和γ干扰素）的水平。
两组均未观察到脑脊液细胞增多。患者组脑脊液白细胞介素-6水平（7.02±5.88 pg/mL）显著高于对照组（1.14±1.90 pg/mL）（p<0.0001）。患者组脑脊液γ干扰素平均水平（24.43±40.16 pg/mL）也显著高于对照组（0.0±0.0 pg/mL）（p<0.0001）。患者组中任何患者（0%）的脑脊液α干扰素均未检测到，但在4例（10.3%）对照组中检测到。仅在患者组的2例患者（8.7%）和对照组的1例患者（2.6%）中检测到β干扰素。脑脊液白细胞介素-6水平与扩散加权磁共振成像上白质病变的程度呈正相关（r=0.607，p=0.002）。
脑脊液中促炎细胞因子水平显著升高，同时I型干扰素检测率极低，表明新生儿轮状病毒相关性白质脑病可能与中枢神经系统炎症过程相关，而无病毒直接侵入中枢神经系统。
