Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, Kunming, 650204, China.
Plant Germplasm and Genomics Center, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China.
Sci Rep. 2017 Nov 17;7(1):15803. doi: 10.1038/s41598-017-16102-0.
Calcium-dependent protein kinases (CDPKs) as crucial sensors of calcium concentration changes play important roles in responding to abiotic and biotic stresses. In this study, 55 BrrCDPK genes, which were phylogenetically clustered into four subfamilies, were identified. Chromosome locations indicated that the CDPK family in turnip expanded by segmental duplication and genome rearrangement. Moreover, gene expression profiles showed that different BrrCDPKs were expressed in specific tissues or stages. Transcript levels of BrrCDPKs indicated that they were involved in abiotic and biotic stresses and that paralogs exhibited functional divergence. Additionally, we identified 15 Rboh genes in turnip; the results of yeast two-hybrid analysis suggested that BrrRbohD1 interacted only with BrrCDPK10 and that BrrRbohD2 interacted with BrrCDPK4/7/9/10/17/22/23. Most of the genes play an important role in pst DC3000 defense by regulating the accumulation of HO and stomatal closure. Our study may provide an important foundation for future functional analysis of BrrCDPKs and reveal further biological roles.
钙依赖蛋白激酶（CDPKs）作为钙浓度变化的关键传感器，在应对非生物和生物胁迫方面发挥着重要作用。本研究鉴定了 55 个 BrrCDPK 基因，它们被分为四个亚家族进行系统发育聚类。染色体定位表明，芜菁的 CDPK 家族通过片段复制和基因组重排而扩张。此外，基因表达谱显示，不同的 BrrCDPK 基因在特定组织或阶段表达。BrrCDPK 基因的转录水平表明它们参与了非生物和生物胁迫，并且旁系同源基因表现出功能分化。此外，我们在芜菁中鉴定了 15 个 Rboh 基因；酵母双杂交分析的结果表明，BrrRbohD1 仅与 BrrCDPK10 相互作用，而 BrrRbohD2 与 BrrCDPK4/7/9/10/17/22/23 相互作用。大多数基因通过调节 HO 的积累和气孔关闭在 pst DC3000 防御中发挥重要作用。我们的研究可能为未来 BrrCDPKs 的功能分析提供重要基础，并揭示其进一步的生物学作用。
