Malucelli Emil, Procopio Alessandra, Fratini Michela, Gianoncelli Alessandra, Notargiacomo Andrea, Merolle Lucia, Sargenti Azzurra, Castiglioni Sara, Cappadone Concettina, Farruggia Giovanna, Lombardo Marco, Lagomarsino Stefano, Maier Jeanette A, Iotti Stefano
Department of Pharmacy and Biotechnology, University of Bologna, 40127, Bologna, Italy.
Museo Storico della Fisica e Centro Studi e Ricerche Enrico Fermi, Piazza del Viminale, 1, 00184, Roma, Italy.
Anal Bioanal Chem. 2018 Jan;410(2):337-348. doi: 10.1007/s00216-017-0725-8. Epub 2017 Nov 17.
The quantification of elemental concentration in cells is usually performed by analytical assays on large populations missing peculiar but important rare cells. The present article aims at comparing the elemental quantification in single cells and cell population in three different cell types using a new approach for single cells elemental analysis performed at sub-micrometer scale combining X-ray fluorescence microscopy and atomic force microscopy. The attention is focused on the light element Mg, exploiting the opportunity to compare the single cell quantification to the cell population analysis carried out by a highly Mg-selective fluorescent chemosensor. The results show that the single cell analysis reveals the same Mg differences found in large population of the different cell strains studied. However, in one of the cell strains, single cell analysis reveals two cells with an exceptionally high intracellular Mg content compared with the other cells of the same strain. The single cell analysis allows mapping Mg and other light elements in whole cells at sub-micrometer scale. A detailed intensity correlation analysis on the two cells with the highest Mg content reveals that Mg subcellular localization correlates with oxygen in a different fashion with respect the other sister cells of the same strain. Graphical abstract Single cells or large population analysis this is the question!
细胞中元素浓度的定量通常通过对大量细胞进行分析测定来完成，而这些细胞群体中缺少特殊但重要的稀有细胞。本文旨在使用一种结合X射线荧光显微镜和原子力显微镜在亚微米尺度上对单细胞进行元素分析的新方法，比较三种不同细胞类型的单细胞和细胞群体中的元素定量。研究重点是轻元素镁，利用这一机会将单细胞定量与通过高度选择性镁荧光化学传感器进行的细胞群体分析进行比较。结果表明，单细胞分析揭示了在所研究的不同细胞系的大量细胞中发现的相同镁差异。然而，在其中一个细胞系中以及其他细胞相比，单细胞分析发现两个细胞的细胞内镁含量异常高。单细胞分析允许在亚微米尺度上绘制整个细胞中的镁和其他轻元素。对镁含量最高的两个细胞进行的详细强度相关性分析表明，与同一细胞系的其他姐妹细胞相比，镁在亚细胞水平上的定位与氧的相关性不同。图形摘要 单细胞还是大量细胞分析，这是个问题！
