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用靶向SATB1的短发夹RNA抑制前列腺癌细胞生长。

Inhibition of prostate cancer cell growth with short hairpin RNA targeting SATB1.

作者信息

Wang Qiang, Hu Shi-Cheng, Yang Chun-Sheng, Chen Jia-Cun, Zheng Jun-Nian, Sun Xiao-Qing, Wang Jun-Qi

机构信息

The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210046, P.R. China.

Department of Urology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221002, P.R. China.

出版信息

Oncol Lett. 2017 Dec;14(6):6592-6596. doi: 10.3892/ol.2017.7006. Epub 2017 Sep 20.

DOI:10.3892/ol.2017.7006
PMID:29151908
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5678242/
Abstract

Despite previous advances, the treatment options for prostate cancer remain limited. For the purposes of gene knockdown, the utility of RNA interference has been demonstrated and is considered to have therapeutic potential. In the present study, a short hairpin RNA (shRNA) was used to assess the effect of special AT-rich sequence binding protein (SATB1) downregulation on the growth and metastatic potential of prostate cancer in xenograft nude mice. A plasmid carrying shRNA targeting SATB1, pSilencer-SATB1-shRNA, was successfully engineered. Using this plasmid, significant downregulation of SATB1 mRNA and protein expression in the DU145 prostate cancer cells was observed. pSilencer-SATB1-shRNA was demonstrated to be markedly efficacious against prostate cancer xenografts in nude mice. These results may lead to a novel method of improving gene therapy efficacy against prostate cancer via regulating the function of SATB1.

摘要

尽管此前已取得进展，但前列腺癌的治疗选择仍然有限。就基因敲低而言，RNA干扰的效用已得到证实，并被认为具有治疗潜力。在本研究中，使用短发夹RNA（shRNA）来评估富含AT序列结合蛋白（SATB1）下调对异种移植裸鼠前列腺癌生长和转移潜能的影响。成功构建了携带靶向SATB1的shRNA的质粒pSilencer-SATB1-shRNA。使用该质粒，观察到DU145前列腺癌细胞中SATB1 mRNA和蛋白表达显著下调。pSilencer-SATB1-shRNA被证明对裸鼠前列腺癌异种移植具有显著疗效。这些结果可能会带来一种通过调节SATB1功能来提高前列腺癌基因治疗疗效的新方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836b/5678242/02a6af881cad/ol-14-06-6592-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836b/5678242/4dd07ac0b071/ol-14-06-6592-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836b/5678242/618a6fe670d5/ol-14-06-6592-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836b/5678242/92112e345236/ol-14-06-6592-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836b/5678242/02a6af881cad/ol-14-06-6592-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836b/5678242/4dd07ac0b071/ol-14-06-6592-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836b/5678242/618a6fe670d5/ol-14-06-6592-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836b/5678242/92112e345236/ol-14-06-6592-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836b/5678242/02a6af881cad/ol-14-06-6592-g03.jpg

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本文引用的文献

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Oncolytic virus carrying shRNA targeting SATB1 inhibits prostate cancer growth and metastasis.携带靶向SATB1的短发夹RNA的溶瘤病毒可抑制前列腺癌的生长和转移。
Tumour Biol. 2015 Nov;36(11):9073-81. doi: 10.1007/s13277-015-3658-x. Epub 2015 Jun 19.
2
Management of early-stage prostate cancer.早期前列腺癌的管理
Am J Manag Care. 2014 Dec;20(12 Suppl):S260-72.
3
RNA interference and its role in cancer therapy.RNA干扰及其在癌症治疗中的作用。
Exp Ther Med. 2018 Mar;15(3):3028-3033. doi: 10.3892/etm.2018.5792. Epub 2018 Jan 24.
Adv Pharm Bull. 2014 Dec;4(4):313-21. doi: 10.5681/apb.2014.046. Epub 2014 Aug 10.
4
RNA interference: A futuristic tool and its therapeutic applications.RNA 干扰：一种未来主义的工具及其治疗应用。
Saudi J Biol Sci. 2012 Oct;19(4):395-403. doi: 10.1016/j.sjbs.2012.08.001. Epub 2012 Aug 17.
5
SATB1 is overexpressed in metastatic prostate cancer and promotes prostate cancer cell growth and invasion.SATB1 在转移性前列腺癌中过表达，促进前列腺癌细胞的生长和侵袭。
J Transl Med. 2013 May 4;11:111. doi: 10.1186/1479-5876-11-111.
6
Cancer statistics, 2013.癌症统计数据，2013 年。
CA Cancer J Clin. 2013 Jan;63(1):11-30. doi: 10.3322/caac.21166. Epub 2013 Jan 17.
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Upregulation of SATB1 is associated with prostate cancer aggressiveness and disease progression.SATB1 的上调与前列腺癌的侵袭性和疾病进展有关。
PLoS One. 2013;8(1):e53527. doi: 10.1371/journal.pone.0053527. Epub 2013 Jan 7.
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