School of Marine Sciences and Biotechnology, Guangxi Key Laboratory of Utilization of Microbial and Botanical Resources, Guangxi University for Nationalities, Nanning, Guangxi 530008, China; National Non-grain Bio-energy Engineering Research Center, Guangxi Academy of Sciences, Nanning, Guangxi 530007, China.
School of Marine Sciences and Biotechnology, Guangxi Key Laboratory of Utilization of Microbial and Botanical Resources, Guangxi University for Nationalities, Nanning, Guangxi 530008, China; Biology Institute, Guangxi Academy of Sciences, Nanning, Guangxi 530007, China.
Bioresour Technol. 2018 Feb;250:35-42. doi: 10.1016/j.biortech.2017.09.208. Epub 2017 Oct 4.
A novel process of enzyme pretreatment and semi-simultaneous saccharification and fermentation (SSSF) was developed in this work to improve succinic acid (SA) productivity from duckweed (Landoltia punctata) and achieve low viscosity. Viscosity (83.86%) was reduced by the pretreatment with combined enzymes at 50 °C for 2 h to a greater extent than that by single enzyme (26.19-71.75%). SSSF was an optimal combination with 65.31 g/L of SA content, which was remarkably higher than those obtained through conventional separate hydrolysis and fermentation (62.12 g/L) and simultaneous saccharification and fermentation (52.41 g/L). The combined approach was effective for SA production. Approximately 75.46 g/L of SA content with a yield of 82.87% and a productivity of 1.35 g/L/h was obtained after 56 h in a 2 L bioreactor. Further studies will focus on increasing the working scale of the proposed method.
本工作开发了一种新型的酶预处理和半同步糖化发酵（SSSF）工艺，以提高浮萍（Landoltia punctata）中琥珀酸（SA）的产量并降低其黏度。与单一酶相比，联合酶在 50°C 下预处理 2 小时可更大程度地降低黏度（83.86%）（26.19-71.75%）。SSSF 是一种最佳的组合，可获得 65.31 g/L 的 SA 含量，明显高于常规的分别水解和发酵（62.12 g/L）和同步糖化和发酵（52.41 g/L）。该联合方法对 SA 的生产有效。在 2L 生物反应器中经过 56 小时后，可获得约 75.46 g/L 的 SA 含量，收率为 82.87%，生产强度为 1.35 g/L/h。进一步的研究将集中在提高所提出方法的工作规模上。
