Seppänen-Laakso Tuulikki, Nygren Heli, Rischer Heiko
VTT Technical Research Centre of Finland Ltd., Espoo, Finland.
Methods Mol Biol. 2020;1980:223-232. doi: 10.1007/7651_2017_109.
Constituents of microalgae and sample preparation for UPLC-ELSD and GC-MS analyses are described. Bound fatty acids from acylglycerols, alkylacylglycerols, galactosyldiacylglycerols, glycerophospholipids, and sterol esters are derivatized by using transesterification with sodium methoxide to form fatty acid methyl esters. Compounds containing free hydroxyl groups, either present originally or formed during previous step, like free fatty acids, sterols, α-tocopherol, phytol, and nonesterified alkoxyglycerols, are trimethylsilylated. The compounds in algal lipid extract are subsequently derivatized by these two steps.
本文描述了微藻的成分以及用于超高效液相色谱-蒸发光散射检测器（UPLC-ELSD）和气相色谱-质谱联用（GC-MS）分析的样品制备方法。来自酰基甘油、烷基酰基甘油、半乳糖基二酰基甘油、甘油磷脂和甾醇酯的结合脂肪酸通过用甲醇钠进行酯交换反应衍生化，形成脂肪酸甲酯。含有游离羟基的化合物，无论是原本存在的还是在上一步中形成的，如游离脂肪酸、甾醇、α-生育酚、叶绿醇和未酯化的烷氧基甘油，都进行三甲基硅烷化。藻类脂质提取物中的化合物随后通过这两个步骤进行衍生化。
