Yang F Q, Feng K, Zhao J, Li S P
Institute of Chinese Medical Sciences, University of Macau, Macao SAR, PR China.
J Pharm Biomed Anal. 2009 Jul 12;49(5):1172-8. doi: 10.1016/j.jpba.2009.02.025. Epub 2009 Mar 9.
Ten free fatty acids namely lauric acid, myristic acid, pentadecanoic acid, palmitoleic acid, palmitic acid, linoleic acid, oleic acid, stearic acid, docosanoic acid and lignoceric acid and four free sterols including ergosterol, cholesterol, campesterol and beta-sitosterol in natural (wild) Cordyceps sinensis, Cordyceps liangshanensis and Cordyceps gunnii, as well as cultured C. sinensis and Cordyceps militaris were first determined using pressurized liquid extraction (PLE), trimethylsilyl (TMS) derivatization and GC-MS analysis. The conditions such as the amount of reagent, temperature and time for TMS derivatization of analytes were optimized. Under the optimum conditions, all calibration curves showed good linearity within the tested ranges. The intra- and inter-day variations for 14 investigated compounds were less than 3.4% and 5.2%, respectively. The results showed that palmitic acid, linoleic acid, oleic acid, stearic acid and ergosterol are main components in natural and cultured Cordyceps which could be discriminated by hierarchical clustering analysis based on the contents of 14 investigated compounds or the 4 fatty acids, where the contents of palmitic acid and oleic acid in natural Cordyceps are significantly higher than those in the cultured ones.
首次采用加压液体萃取（PLE）、三甲基硅烷基（TMS）衍生化和气相色谱 - 质谱联用（GC-MS）分析方法，测定了天然（野生）冬虫夏草、凉山虫草、古尼虫草以及人工培养的冬虫夏草和蛹虫草中的10种游离脂肪酸，即月桂酸、肉豆蔻酸、十五烷酸、棕榈油酸、棕榈酸、亚油酸、油酸、硬脂酸、二十二烷酸和木蜡酸，以及4种游离甾醇，即麦角甾醇、胆固醇、菜油甾醇和β-谷甾醇。对分析物TMS衍生化的试剂用量、温度和时间等条件进行了优化。在最佳条件下，所有校准曲线在测试范围内均呈现良好的线性关系。14种被测化合物的日内和日间变化分别小于3.4%和5.2%。结果表明，棕榈酸、亚油酸、油酸、硬脂酸和麦角甾醇是天然和人工培养冬虫夏草中的主要成分，基于14种被测化合物或4种脂肪酸的含量，通过层次聚类分析可以区分它们，其中天然冬虫夏草中棕榈酸和油酸的含量显著高于人工培养的冬虫夏草。
