Niemhom Nantawan, Thawai Chitti
Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
Actinobacterial Research Unit, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
Int J Syst Evol Microbiol. 2018 Jan;68(1):294-298. doi: 10.1099/ijsem.0.002503. Epub 2017 Nov 21.
A novel actinomycete strain PS42-9, which formed short chains of spores borne on the tip of long sporophores arising from the substrate mycelium, was isolated from soil in Phu-Sang National Park, Phayao province, Thailand. The isolate contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell sugars of strain PS42-9 were glucose, madurose, mannose, rhamnose and ribose. The characteristic phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxyphosphatidylethanolamine and ninhydrin-positive glycophospholipids. The major menaquinone was MK-10(H4). The main cellular fatty acids were C17 : 1ω8c and C17 : 0. The G+C content of the genomic DNA was 71.5 mol%. Phylogenetic analysis using 16S rRNA gene sequences revealed that strain PS42-9 should be classified in the genus Herbidospora and was closely related to Herbidospora sakaeratensis DMKUA 205 (99.10 %) and Herbidospora yilanensis NBRC 106371 (98.61 %). The result of DNA-DNA hybridization and some physiological and biochemical properties indicated that strain PS42-9 could be readily distinguished from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name Herbidospora soli sp. nov. is proposed. The type strain is PS42-9 (=BCC 46909=NBRC 108780).
从泰国帕尧府普桑国家公园的土壤中分离出一株新型放线菌菌株PS42-9，该菌株形成短链孢子，着生于由基质菌丝体产生的长孢子梗顶端。该分离株在细胞壁肽聚糖中含有内消旋二氨基庚二酸。菌株PS42-9的全细胞糖包括葡萄糖、马杜拉糖、甘露糖、鼠李糖和核糖。其特征性磷脂为磷脂酰乙醇胺、磷脂酰甲基乙醇胺、羟基磷脂酰乙醇胺和茚三酮阳性糖脂。主要的甲基萘醌是MK-10(H4)。主要的细胞脂肪酸是C17 : 1ω8c和C17 : 0。基因组DNA的G+C含量为71.5 mol%。使用16S rRNA基因序列进行的系统发育分析表明，菌株PS42-9应归类于草孢菌属，与坂叻草孢菌DMKUA 205（99.10 %）和宜兰草孢菌NBRC 106371（98.61 %）密切相关。DNA-DNA杂交结果以及一些生理生化特性表明，菌株PS42-9可与其最亲近的系统发育亲属轻易区分开来。基于这些表型和基因型数据，该菌株代表一个新物种，为此提出名为土壤草孢菌（Herbidospora soli sp. nov.）的名称。模式菌株为PS42-9（=BCC 46909=NBRC 108780）。
