Yu Mingying, Zhou Rui, Li Jingjing, Han Liyuan, Wang Han, Zhang Siyu, Zhao Junwei, Wang Xiangjing, Song Jia, Xiang Wensheng
Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People's Republic of China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
Antonie Van Leeuwenhoek. 2021 May;114(5):581-590. doi: 10.1007/s10482-021-01542-6. Epub 2021 Mar 1.
An aerobic, non-motile, Gram-stain positive, cellulose-degrading actinobacterium, designated strain NEAU-GS84, was isolated from a forest soil sample collected from Linchun Ridge Forest Park in Sanya, Hainan Province, China, and characterized using a polyphasic approach. Morphological and chemotaxonomic characteristics indicated that strain NEAU-GS84 could belong to the genus Herbidospora. The 16S rRNA gene sequence analysis indeed confirmed that strain NEAU-GS84 belonged to the genus Herbidospora and was most closely related to Herbidospora yilanensis JCM 18062 (99.2% 16S rRNA gene sequence similarity) and Herbidospora galbida NEAU-GS14 (99.0%). The cell wall of strain NEAU-GS84 contained meso-diaminopimelic acid as the major diamino acid and the whole-cell hydrolysates mainly contained glucose, madurose and ribose. The major polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyphosphatidylethanolamin, phosphoglycolipids, two phosphatidylinositol mannosides and one unidentified phospholipid. The predominant menaquinone was MK-10(H). Major fatty acids were 10-methly C, C and iso-C. These chemotaxonomic data substantiated the affiliation of strain NEAU-GS84 to the genus Herbidospora. The DNA G+C content was 70.7 mol%. The genome size of strain NEAU-GS84 is about 8.37 Mb and contained 41 cellulose-binding domain synthesis genes, 13 β-glucosidase synthesis genes, 6 endoglucanase synthesis genes and 9 xylanase synthesis genes. Based on digital DNA-DNA hybridization and average nucleotide identity values, the new strain NEAU-GS84 could be differentiated from its closest relatives. Therefore, the strain represents a novel species of the genus Herbidospora, for which the name Herbidospora solisilvae sp. nov. is proposed. The type strain is NEAU-GS84 (= CCTCC AA 2018041 = JCM 33460).
从中国海南省三亚市临春岭森林公园采集的森林土壤样本中分离出一株需氧、不运动、革兰氏染色阳性、可降解纤维素的放线菌，命名为菌株NEAU-GS84，并采用多相分类法对其进行了表征。形态学和化学分类学特征表明，菌株NEAU-GS84可能属于Herbidospora属。16S rRNA基因序列分析确实证实，菌株NEAU-GS84属于Herbidospora属，与伊兰Herbidospora yilanensis JCM 18062（16S rRNA基因序列相似性为99.2%）和微黄Herbidospora galbida NEAU-GS14（99.0%）关系最为密切。菌株NEAU-GS84的细胞壁含有内消旋二氨基庚二酸作为主要二氨基酸，全细胞水解产物主要含有葡萄糖、马杜拉糖和核糖。主要极性脂为双磷脂酰甘油、磷脂酰甲基乙醇胺、磷脂酰乙醇胺、羟基磷脂酰乙醇胺、磷酸糖脂、两种磷脂酰肌醇甘露糖苷和一种未鉴定的磷脂。主要甲基萘醌为MK-10(H)。主要脂肪酸为10-甲基C、C和异-C。这些化学分类学数据证实了菌株NEAU-GS84属于Herbidospora属。DNA G+C含量为70.7 mol%。菌株NEAU-GS84的基因组大小约为8.37 Mb，包含41个纤维素结合结构域合成基因、13个β-葡萄糖苷酶合成基因、6个内切葡聚糖酶合成基因和9个木聚糖酶合成基因。基于数字DNA-DNA杂交和平均核苷酸同一性值，新菌株NEAU-GS84可与其最接近的亲缘种区分开来。因此，该菌株代表Herbidospora属的一个新物种，为此提出名为Herbidospora solisilvae sp. nov.的名称。模式菌株为NEAU-GS84（=CCTCC AA 2018041=JCM 33460）。
