Thawai Chitti, He Ya-Wen, Tadtong Sarin
1Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
2Actinobacterial Research Unit, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
Int J Syst Evol Microbiol. 2018 Oct;68(10):3345-3350. doi: 10.1099/ijsem.0.002998. Epub 2018 Aug 31.
A novel actinomycete, strain PLAI 1-1, which formed spiny single spore directly on substrate mycelium was isolated from root tissue of Zingiber montanum. The isolate contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid in the cell-wall peptidoglycan. The acyl type of the cell-wall muramic acid was glycolyl. The whole-cell sugars of strain PLAI 1-1 were glucose, arabinose, xylose, ribose and a trace amount of mannose. The membrane phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine and phosphatidylinositol. The major menaquinone was MK-9 (H4). The main cellular fatty acids were iso-C15 : 0 and C17 : 1ω8c. The G+C content of the genomic DNA was 70.6 mol%. 16S rRNA gene sequence analysis revealed that strain PLAI 1-1 was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430 (99.2 %). Based on the data of physiological and biochemical tests, including the result of DNA-DNA hybridization, strain PLAI 1-1 represents a novel species of the genus Jishengella, for which the name Jishengellazingiberis sp. nov. is proposed. The type strain is PLAI 1-1 (=TBRC 7644=NBRC 113144).
从高良姜根组织中分离出一株新型放线菌，菌株PLAI 1-1，它直接在基内菌丝体上形成带刺的单孢子。该分离株在细胞壁肽聚糖中含有内消旋二氨基庚二酸和3-羟基二氨基庚二酸。细胞壁胞壁酸的酰基类型为糖基。菌株PLAI 1-1的全细胞糖为葡萄糖、阿拉伯糖、木糖、核糖和微量甘露糖。膜磷脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰丝氨酸和磷脂酰肌醇。主要的甲基萘醌是MK-9（H4）。主要的细胞脂肪酸是异-C15 : 0和C17 : 1ω8c。基因组DNA的G+C含量为70.6摩尔%。16S rRNA基因序列分析表明，菌株PLAI 1-1是吉生氏菌属的成员，与内生吉生氏菌DSM 45430的16S rRNA基因序列相似度最高（99.2%）。基于生理生化测试数据，包括DNA-DNA杂交结果，菌株PLAI 1-1代表吉生氏菌属的一个新物种，为此提出名称为内生吉生氏菌（Jishengellazingiberis sp. nov.）。模式菌株是PLAI 1-1（=TBRC 7644=NBRC 113144）。
