Song Chi, Chen Qiang, Wu Xiangli, Zhang Jinxia, Huang Chenyang
Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Curr Microbiol. 2014 Nov;69(5):611-6. doi: 10.1007/s00284-014-0634-4. Epub 2014 Jun 18.
High temperature is an important environmental factor that affects the growth and development of most edible fungi, however, the mechanism(s) for resistance to high temperature remains elusive. Nitric oxide is known to be able to effectively alleviate oxidative damage and plays an important role in the regulation of trehalose accumulation during heat stress in mycelia of Pleurotus eryngii var. tuoliensis. In this paper, we investigated whether heat stress can activate apoptosis-like cell death in mycelia of Pleurotus. Two Pleurotus species were used to detect morphological features characteristic of apoptosis including nuclear condensation, reactive oxygen species accumulation, and DNA fragmentation when exposed to heat stress (42 °C). The results showed that these classical apoptosis markers were apparent in Pleurotus strains after heat treatment. The heat-induced apoptosis-like cell death in Pleurotus was further probed using oligomycin and N-acetylcysteine, both of which were shown to block processes leading to apoptosis. This is the first report that apoptosis-like cell death occurs in Pleurotus species as a result of abiotic stress, and that this process can be inhibited with chemicals that block mitochondrial-induced apoptotic pathways and/or with ROS-scavenging compounds.
高温是影响大多数食用菌生长发育的重要环境因素，然而，其耐高温机制仍不清楚。已知一氧化氮能够有效减轻氧化损伤，并在刺芹侧耳变种菌丝体热胁迫期间海藻糖积累的调节中发挥重要作用。在本文中，我们研究了热胁迫是否能激活侧耳属菌丝体中类似凋亡的细胞死亡。使用两种侧耳属物种检测暴露于热胁迫（42°C）时凋亡的形态学特征，包括核浓缩、活性氧积累和DNA片段化。结果表明，这些经典的凋亡标记在热处理后的侧耳属菌株中很明显。使用寡霉素和N-乙酰半胱氨酸进一步探究了侧耳属中热诱导的类似凋亡的细胞死亡，二者均显示出可阻断导致凋亡的过程。这是首次报道侧耳属物种因非生物胁迫而发生类似凋亡的细胞死亡，并且该过程可被阻断线粒体诱导的凋亡途径的化学物质和/或活性氧清除化合物所抑制。
