Department of Organic Chemistry, University of Cordoba (UCO), Cordoba 14014, Spain.
Seneca Green Catalyst S.L., Rabanales XXI/University of Cordoba, Cordoba 14014, Spain.
Molecules. 2017 Nov 21;22(11):2025. doi: 10.3390/molecules22112025.
The application of biocatalysts in the transesterification process of triglycerides (TG) allows integrating the glycerol in the form of monoglyceride (MG), sharply increasing the yield and the environmental sustainability of the conventional biodiesel production process. This is known as Ecodiesel. To overcome the inconvenient of the high cost of the currently employed highly purified commercial enzymes, the use of scarcely purified extracts obtained from standard strains of the same species of commercial lipases currently applied in this process is being investigated. Thus, type B (CALB) was chosen to determine the optimal conditions of culture of this yeast. The standard strain was obtained from the Spanish Type Microbial Cultures Collection (CECT) and has been used to carry out several studies to elucidate its optimum growth conditions. Through a process of lyophilization with prior dialysis of the liquid cultures, the enzymatic extracts were obtained. The different obtained cultures have been applied as biocatalysts in the 1,3-selective transesterification reaction of sunflower oil with ethanol to obtain Ecodiesel (FAEE + MG). Selectivity and reaction yields were obtained by gas chromatography. Acceptable yields are obtained during the reaction time as well as in successive reactions, demonstrating the feasibility of using these CALB enzymatic extracts as biocatalysts.
生物催化剂在甘油三酯（TG）的酯交换过程中的应用允许以单甘酯（MG）的形式整合甘油，大大提高了传统生物柴油生产过程的产率和环境可持续性。这被称为生态柴油。为了克服目前使用的高纯度商业酶成本高的不便，正在研究使用从标准菌株中获得的几乎未纯化的提取物，这些标准菌株是目前应用于该过程的商业脂肪酶的同一种。因此，选择了类型 B（CALB）来确定该酵母的最佳培养条件。标准菌株来自西班牙类型微生物培养物收藏（CECT），并已用于进行多项研究以阐明其最佳生长条件。通过在液体培养物预先透析后进行冻干，获得了酶提取物。将不同获得的培养物用作生物催化剂，用于葵花籽油与乙醇的 1,3-选择性酯交换反应，以获得生态柴油（FAEE + MG）。通过气相色谱法获得选择性和反应产率。在反应时间内以及连续反应中均获得了可接受的产率，证明了使用这些 CALB 酶提取物作为生物催化剂的可行性。
