Department of Molecular Medicine, Rudjer Boskovic Institute, Bijenicka 54, 10000 Zagreb, Croatia.
Environ Toxicol. 2012 Nov;27(11):644-52. doi: 10.1002/tox.20683. Epub 2011 Mar 3.
Acrolein is a toxic unsaturated aldehyde and widespread environmental pollutant produced during lipid peroxidation and also by burning of tobacco or liquid fuels. Inhalation or dermal exposure to acrolein could be toxic to organisms. This very reactive aldehyde has a strong affinity for binding to proteins thus forming pathogenic protein-adducts. In the present study we have analyzed formation of bioreactive acrolein-protein adducts in bovine serum albumin solution exposed to exhaust gases of mineral diesel fuel and of mineral diesel fuel supplemented with different amounts of a novel diesel fuel additive denoted Ecodiesel (produced by a genuine procedure of recycling of plant oils used for food preparation). The effects of acrolein-protein adducts were tested on human microvascular endothelial cells and on human osteosarcoma cells that are sensitive to bioactivities of lipid peroxidation products. The results have shown a reduction of the bioreactive acrolein in exhaust gases when mineral diesel was supplemented with 5-20% Ecodiesel. Moreover, acrolein-protein adducts obtained from mineral diesel supplemented with Ecodiesel were less toxic than those obtained from mineral diesel alone. Thus, we assume that supplementing mineral diesel fuel with Ecodiesel would be of benefit for the use of renewable energy, for environment and for human health due to reduced environmental pollution with bioreactive acrolein.
丙烯醛是一种有毒的不饱和醛，广泛存在于脂质过氧化过程中，也是烟草或液体燃料燃烧时产生的污染物。丙烯醛的吸入或皮肤接触对生物体可能是有毒的。这种非常活泼的醛类具有很强的与蛋白质结合的亲和力，从而形成致病的蛋白质加合物。在本研究中，我们分析了在暴露于矿物柴油废气和添加不同量新型柴油燃料添加剂 Ecodiesel（通过真正的植物油回收程序生产，用于食品制备）的矿物柴油的牛血清白蛋白溶液中形成的生物反应性丙烯醛-蛋白质加合物。将丙烯醛-蛋白质加合物作用于对脂质过氧化产物的生物活性敏感的人微血管内皮细胞和人骨肉瘤细胞。结果表明，当矿物柴油中添加 5-20%的 Ecodiesel 时，废气中的生物反应性丙烯醛减少。此外，从添加 Ecodiesel 的矿物柴油中获得的丙烯醛-蛋白质加合物比从单独的矿物柴油中获得的丙烯醛-蛋白质加合物毒性更小。因此，我们假设由于生物反应性丙烯醛的环境污染减少，矿物柴油燃料中添加 Ecodiesel 将有利于可再生能源的使用、环境和人类健康。
