市售抗体检测 ELISA 试剂盒检测猪囊尾蚴病的敏感性低且常发生交叉反应。
Low sensitivity and frequent cross-reactions in commercially available antibody detection ELISA assays for Taenia solium cysticercosis.
机构信息
Cysticercosis Unit, Department of Transmissible Diseases, Instituto Nacional de Ciencias Neurologicas, Lima, Peru.
Department of Microbiology, School of Sciences, Universidad Peruana Cayetano Heredia, Lima, Perú.
出版信息
Trop Med Int Health. 2018 Jan;23(1):101-105. doi: 10.1111/tmi.13010. Epub 2017 Dec 7.
OBJECTIVE
To evaluate the diagnostic performance of two commercially available ELISA kits, Novalisa and Ridascreen , for the detection of antibodies to Taenia solium, compared to serological diagnosis of neurocysticercosis (NCC) by LLGP-EITB (electro-immunotransfer blot assay using lentil-lectin purified glycoprotein antigens).
METHODS
Archive serum samples from patients with viable NCC (n = 45) or resolved, calcified NCC (n = 45), as well as sera from patients with other cestode parasites (hymenolepiasis, n = 45 and cystic hydatid disease, n = 45), were evaluated for cysticercosis antibody detection using two ELISA kits, Novalisa and Ridascreen . All NCC samples had previously tested positive, and all samples from heterologous infections were negative on LLGP-EITB for cysticercosis. Positive rates were calculated by kit and sample group and compared between the two kits.
RESULTS
Compared to LLGP-EITB, the sensitivity of both ELISA assays to detect specific antibodies in patients with viable NCC was low (44.4% and 22.2%), and for calcified NCC, it was only 6.7% and 4.5%. Sera from patients with cystic hydatid disease were highly cross-reactive in both ELISA assays (38/45, 84.4%; and 25/45, 55.6%). Sera from patients with hymenolepiasis cross-reacted in five cases in one of the assays (11.1%) and in only one sample with the second assay (2.2%).
CONCLUSIONS
The performance of Novalisa and Ridascreen was poor. Antibody ELISA detection cannot be recommended for the diagnosis of neurocysticercosis.
目的
评估两种市售 ELISA 试剂盒（Novalisa 和 Ridascreen）在检测抗猪带绦虫抗体方面的诊断性能，与使用扁豆凝集素纯化糖蛋白抗原的 LLGP-EITB（电免疫转移印迹分析）进行的神经囊尾蚴病（NCC）血清学诊断相比。
方法
评估来自活 NCC（n=45）或已解决的钙化 NCC（n=45）患者的存档血清样本以及来自其他带绦虫寄生虫感染患者（包虫病，n=45 和囊性Hydatid 病，n=45）的血清样本，使用两种 ELISA 试剂盒（Novalisa 和 Ridascreen）检测囊尾蚴病抗体。所有 NCC 样本先前均检测为阳性，所有异源感染样本在 LLGP-EITB 上对囊尾蚴病检测均为阴性。通过试剂盒和样本组计算阳性率，并比较两种试剂盒之间的差异。
结果
与 LLGP-EITB 相比，两种 ELISA 检测方法在检测活 NCC 患者特异性抗体方面的敏感性均较低（44.4%和 22.2%），而在钙化 NCC 患者中，仅为 6.7%和 4.5%。囊性 Hydatid 病患者的血清在两种 ELISA 检测中均具有高度交叉反应性（38/45，84.4%；和 25/45，55.6%）。在一种 ELISA 检测中，5 例肝片吸虫病患者的血清存在交叉反应（11.1%），而在第二种 ELISA 检测中仅 1 例样本存在交叉反应（2.2%）。
结论
Novalisa 和 Ridascreen 的性能不佳。抗体 ELISA 检测不能用于诊断神经囊尾蚴病。
Zotero 插件