CUNY Graduate School of Public Health and Health Policy and Institute for Implementation Science in Population Health, City University of New York, New York, NY, 10027, USA.
School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, Hong Kong SAR.
Parasitol Res. 2020 Aug;119(8):2521-2529. doi: 10.1007/s00436-020-06750-z. Epub 2020 Jun 26.
The enzyme-linked immunoelectrotransfer blot (EITB) assay to detect antibodies in serum is a complementary tool for the diagnosis of neurocysticercosis (NCC). Presence of at least one glycoprotein band corresponding to a Taenia solium (T. solium) antigen indicates a positive result; however, EITB assays have multiple glycoprotein bands, and previous work has suggested that band patterns may have additional diagnostic value. We included 58 participants with a definitive diagnosis of NCC who received care at the Instituto Nacional de Neurología y Neurocirugía in Mexico City. Three different EITB tests were applied to participants' serum samples (LDBio, France; US Centers for Disease Control and Prevention [CDC]; and Instituto de Diagnóstico y Referencia Epidemiológicos [InDRE]). There was substantial variability in specific glycoprotein band patterns among the three assays. However, in age- and sex-adjusted logistic regression models, the number of glycoprotein bands was positively associated with the presence of vesicular extraparenchymal cysts (InDRE adjusted odds ratio [aOR] 1.60 p < 0.001; CDC aOR 6.31 p < 0.001; LDBio aOR 2.45 p < 0.001) and negatively associated with the presence of calcified parenchymal cysts (InDRE aOR 0.63 p < 0.001; CDC aOR 0.25 p < 0.001; LDBio aOR 0.44 p < 0.001). In a sensitivity analysis also adjusting for cyst count, results were similar. In all three EITB serum antibody tests, the number of glycoprotein bands consistently predicted cyst stage and location, although magnitude of effect differed.
酶联免疫电泳转移印迹（EITB）检测血清抗体是诊断囊尾蚴病（NCC）的辅助工具。至少存在一条对应于猪带绦虫（T. solium）抗原的糖蛋白带表明结果为阳性；然而，EITB 检测有多个糖蛋白带，先前的研究表明带型可能具有额外的诊断价值。我们纳入了 58 名在墨西哥城国立神经病学和神经外科学研究所接受治疗的明确诊断为 NCC 的参与者。对参与者的血清样本应用了三种不同的 EITB 检测（法国 LDBio；美国疾病控制与预防中心 [CDC]；和 Instituto de Diagnóstico y Referencia Epidemiológicos [InDRE]）。在这三种检测中，特定糖蛋白带型存在很大差异。然而，在年龄和性别调整的逻辑回归模型中，糖蛋白带的数量与囊泡性脑外囊虫病的存在呈正相关（InDRE 调整后的优势比 [aOR] 1.60，p<0.001；CDC aOR 6.31，p<0.001；LDBio aOR 2.45，p<0.001），与脑实质钙化囊虫病的存在呈负相关（InDRE aOR 0.63，p<0.001；CDC aOR 0.25，p<0.001；LDBio aOR 0.44，p<0.001）。在还调整了囊虫计数的敏感性分析中，结果相似。在所有三种 EITB 血清抗体检测中，糖蛋白带的数量一致预测了囊虫病的分期和位置，尽管影响的程度有所不同。
