Pégard Nicolas C, Toth Marton L, Driscoll Monica, Fleischer Jason W
Princeton University, Department of Electrical Engineering, Olden Street, Princeton, NJ 08544, USA.
Lab Chip. 2014 Dec 7;14(23):4447-50. doi: 10.1039/c4lc00701h. Epub 2014 Sep 26.
We present a 3D tomography technique for in vivo observation of microscopic samples. The method combines flow in a microfluidic channel, illumination through a slit aperture, and a Fourier lens for simultaneous acquisition of multiple perspective angles in the phase-space domain. The technique is non-invasive and naturally robust to parasitic sample motion. 3D absorption is retrieved using standard back-projection algorithms, here a limited-domain inverse radon transform. Simultaneously, 3D differential phase contrast images are obtained by computational refocusing and comparison of complementary illumination angles. We implement the technique on a modified glass slide which can be mounted directly on existing optical microscopes. We demonstrate both amplitude and phase tomography on live, freely swimming C. elegans nematodes.
我们提出了一种用于体内观察微观样本的三维断层扫描技术。该方法将微流控通道中的流动、通过狭缝孔径的照明以及用于在相空间域中同时获取多个视角的傅里叶透镜相结合。该技术是非侵入性的，并且对寄生样本运动具有天然的鲁棒性。使用标准的反投影算法（这里是有限域逆拉东变换）来恢复三维吸收。同时，通过计算重聚焦和互补照明角度的比较获得三维微分相衬图像。我们在一个可以直接安装在现有光学显微镜上的改良载玻片上实现了该技术。我们展示了对活的、自由游动的秀丽隐杆线虫的幅度和相位断层扫描。
