Frosini Sian-Marie, Bond Ross
Clinical Science and Services, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield AL9 7TA, UK.
Antibiotics (Basel). 2017 Nov 22;6(4):29. doi: 10.3390/antibiotics6040029.
Emergence of multidrug-resistance in (SP) has increased interest in topical therapy as an alternative to systemic antibiotics in canine pyoderma. The antifungal imidazole, clotrimazole, is contained in numerous licensed canine ear preparations. Its in vitro activity against SP has not been evaluated, although previous studies have shown that the related imidazole, miconazole, has significant anti-staphylococcal efficacy. We therefore determined minimum inhibitory concentrations (MICs) of clotrimazole amongst 50 SP isolates (25 methicillin-resistant [MR]SP and susceptible [MS]SP) collected from dogs in Germany during 2010-2011 using an agar dilution method (CLSI VET01-A4). MICs amongst MRSP and MSSP were comparable (MIC and MIC = 1mg/L for both groups, = 0.317); overall, 49 isolates had MIC = 1 mg/L and one had MIC = 0.5 mg/L. The relatively low MICs obtained in this study are likely to be exceeded by topical therapy and thus further clinical evaluation of clotrimazole use in canine superficial pyoderma and otitis externa caused by MRSP and MSSP is now warranted.
犬脓皮病中耐多药葡萄球菌（SP）的出现，使得局部治疗作为全身用抗生素的替代方法治疗犬脓皮病的关注度增加。抗真菌咪唑类药物克霉唑存在于众多已获许可的犬用耳部制剂中。尽管先前的研究表明相关咪唑类药物咪康唑具有显著的抗葡萄球菌功效，但其对SP的体外活性尚未得到评估。因此，我们采用琼脂稀释法（CLSI VET01 - A4）测定了2010 - 2011年从德国犬只中收集的50株SP分离株（25株耐甲氧西林葡萄球菌[MR]SP和敏感[MS]SP）对克霉唑的最低抑菌浓度（MIC）。MRSP和MSSP的MIC相当（两组的MIC和MIC均为1mg/L，= 0.317）；总体而言，49株分离株的MIC = 1mg/L，1株的MIC = 0.5mg/L。本研究中获得的相对较低的MIC可能会被局部治疗所超过，因此现在有必要对克霉唑用于由MRSP和MSSP引起的犬浅表脓皮病和外耳道炎进行进一步的临床评估。
