Saurabh Saumya, Maji Suvrajit, Bruchez Marcel P
Department of Chemistry, Carnegie Mellon University, 4400 Fifth Ave, Pittsburgh, Pennsylvania 15213, USA.
Opt Express. 2012 Mar 26;20(7):7338-49. doi: 10.1364/OE.20.007338.
The ability to detect single molecules over the electronic noise requires high performance detector systems. Electron Multiplying Charge-Coupled Device (EMCCD) cameras have been employed successfully to image single molecules. Recently, scientific Complementary Metal Oxide Semiconductor (sCMOS) based cameras have been introduced with very low read noise at faster read out rates, smaller pixel sizes and a lower price compared to EMCCD cameras. In this study, we have compared the two technologies using two EMCCD and three sCMOS cameras to detect single Cy5 molecules. Our findings indicate that the sCMOS cameras perform similar to EMCCD cameras for detecting and localizing single Cy5 molecules.
在电子噪声背景下检测单分子的能力需要高性能的探测器系统。电子倍增电荷耦合器件（EMCCD）相机已成功用于单分子成像。最近，基于科学互补金属氧化物半导体（sCMOS）的相机被引入，与EMCCD相机相比，其具有极低的读出噪声、更快的读出速率、更小的像素尺寸和更低的价格。在本研究中，我们使用两台EMCCD相机和三台sCMOS相机比较了这两种技术来检测单个Cy5分子。我们的研究结果表明，在检测和定位单个Cy5分子方面，sCMOS相机的性能与EMCCD相机相似。
